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United States Patent | 3,830,222 |
Chance | August 20, 1974 |
A system for providing flash photolysis activation of CO-inhibited cytochrome oxidase in living tissue in the presence of oxygen. In a typical procedure employing cardiac tissue this initiates oxidation of reduced pyridine nucleotide (PN) and flavoprotein (Fp), with a high rate of response. The fractional extent of the photolysis response of PN and Fp indicates the fraction of the total mitochondrial population containing cytochrome a.sub.3 CO to which oxygen has diffused at the time of the photolysis flash, thereby providing an indication of the effectiveness of oxygen diffusion in the tissue without destruction of the tissue. A double flash is used to evaluate the extent of photolysis, one flash occurring a few seconds after perfusing the tissue with oxygen, followed by another flash a few seconds later. The readout is obtained on a storage oscilloscope, using a double beam, time-shared photometer assembly with a compensating photomultiplier. The flash lamps are triggered by a pulse from the compensating photomultiplier, with a delay to fire the flash lamps at an appropriate phase angle.
Inventors: | Chance; Britton (Philadelphia, PA) |
Appl. No.: | 05/269,580 |
Filed: | July 7, 1972 |
Current U.S. Class: | 600/334 ; 356/39 |
Current International Class: | A61B 5/15 (20060101); G01N 21/63 (20060101); A61b 005/00 () |
Field of Search: | 128/2A,2R,2.1R,2.1Z 424/9 356/39 |
3313290 | April 1967 | Chance et al. |
3722504 | March 1973 | Sawyer |
Chance, B. et al. Arch. of Biochem. & Biophysics, Vol. 143, No. 2, April 1971 pp. 675-687. . Chance, B. et al. IEEE Trans. On Bio-Med. Engineering, Vol. 2, April 1970, pp. 118-121. . Chance, B. et al. Review of Sci. Instruments, Vol. 42, No. 7, July 1971, pp. 951-957. . Chance, B., Journ. Of Biological Chemistry, Vol. 202, No. 1, May 1953, pp. 407-416.. |