( 33 of 33 ) |
United States Patent | 4,816,388 |
Sipe , et al. | March 28, 1989 |
Recombinant human prealbumin and a method for producing such human prealbumin through recombinant DNA techniques including preparation and isolation of a cDNA sequence coding for human prealbumin, construction of a cloning vector and an expression vector containing said cDNA sequence, and expression of human prealbumin in a prokaryotic cell transformed by said expression vector are disclosed. The invention further relates to the use of human prealbumin cDNA in the diagnosis by hybridization methodologies of medical conditions with which variant forms of prealbumin are associated. Also disclosed is a method for diagnosing some Type I familial amyloid polyneuropathies by a restriction endonuclease assay with an enzyme which recognizes the nucleotide base sequence 5'-ATGCAT-3'.
Current U.S. Class: | 435/6 ; 435/252.33; 435/320.1; 435/69.6; 435/803; 436/808; 436/811; 536/23.5; 536/24.2; 536/24.3; 536/24.31 |
Current International Class: | C07K 14/435 (20060101); C07K 14/76 (20060101); C12Q 1/68 (20060101); C12Q 001/68 (); C07H 015/12 (); C12N 015/00 () |
Field of Search: | 435/6,68,172.3,253,317,803,810 436/501,808,811 536/27 935/2,4,11,29,73,78,80 |
4395486 | July 1983 | Wilson et al. |
4517294 | May 1985 | Bock et al. |
Sasaki, H., et al., "Diagnosis of Familial Amyloidotic . . . ", Biochem, Biophys. Res. Comm. 125 (2), 636-642 (12/14/84). . Wallace, M. R. et al., "Localization of the Human Prealbumin Gene", Biochem. Biophys. Res. Comm. 129 (3), 753-758 (6/28/85). . Tzuzuke, T., et al., "Structure of the Human Prealbumin Gene", J. Biol. Chem. 260 (22), 12224-12227 (10/5/85). . Benson, M. D., et al., J. Clin. Invest. (1985) 75:71-75. . Whitehead et al., Mol. Biol. Med. (1985) 7: in Press. . Wallace et al., Clin. Res. (1985) 33:592A. . Nakazato, M. et al., The Lancet (1985) 1:99. . Mita, S. et al., Biochemical and Biophysical Research Coomunications Academic Press, Inc., vol. 124, No. 2, 1984, pp. 558-564.. |