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United States Patent | 4,396,713 |
Simpson , et al. | August 2, 1983 |
Hemoflagellates are characterized by restriction endonuclease digestion of the mitochondrial DNA to provide for substantial cleavage of the kDNA network. The resulting electrophoretic profile of the digest can be used as a restriction fingerprint for distinguishing organisms and specific strains. The kDNA is found to be sufficiently stable through numerous passages of the organism to provide a reliable fingerprint.
Inventors: | Simpson; Larry P. (Los Angeles, CA), Morel; Carlos M. (Rio de Janeiro, BR) |
Assignee: |
The Regents of the University of Calif.
(Berkeley,
CA)
|
Appl. No.: | 06/206,200 |
Filed: | November 12, 1980 |
Current U.S. Class: | 435/6 ; 435/18; 435/34; 435/803; 435/810; 435/91.53; 435/92 |
Current International Class: | C12Q 1/68 (20060101); C12Q 001/68 (); C12Q 001/34 (); C12Q 001/04 (); C12P 019/34 () |
Field of Search: | 455/46,34,89,90,91,92,172,803,810,18 23/23B 424/1,1.5 |
Mattei et al., "Biochemical Strain Characterization of Trypanosoma Cruzi by Restriction Endonuclease Cleavage of Kinetoplast--DNA" FEBS. Letters, vol 74, No. 2 (1977) pp. 264-268. . Borst, et al., "Characterization of DNA from Tyrpanosoma Brucei and Related Trypanosomes by Restriction Endonuclease Digestion", Chem. Abstracts, vol 93, No. 21 (1980), p. 221, Nos. 199423p. . Simpson et al., "Replication and Transcription of Kinetoplast DNA", Chem. Abstracts, vol. 92, No. 17, (1980), p. 275 Abts. No. 143068p. . Riou, et al., "Heterogeneity of the Kinetoplast DNA molecules of Trypanosoma Cruzi", Chem. Absts., vol. 87, No. 5 (1977) p. 120 Absts. No. 34600g. . Brack, et al., "The use of Restriction Endonucleases for the Investigation of Kinetoplast DNA", Chem. Absts. vol. 87, No. 9, (1977), p. 269, Absts. No. 65075u. . Frasch, et al. "The Kinetoplast DNA of Trypanosoma Equiperdum", Biochim. Biophys., Acta, vol. 607 (1980), pp. 397-410. . Williams, et al., "Genomic Rearrangements Correluted with Antigenia Variaton in Trypanosoma Lovercsi" Nature 282 (1979) pp. 847-849. . Mattei, et al. "Biochemical Strain Characterization of Trypanosoma Cruzi, by Restriction Endonuclease Cleavage of Kinetoplast DNA", Chem. Absts., vol. 86 No. 19 (1977) p. 267, Abs. No. 136213(e). . Donelson, et al., "Kinetoplast DNA Minicircles of Trypanosoma Brucei Share Regions of Sequence Homology," Chem. Abstracts, vol. 93 No. 25 (1980) p. 936, Absts. No. 234674v.. |