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United States Patent | 4,668,476 |
Bridgham , et al. | May 26, 1987 |
An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another.
Inventors: | Bridgham; John (Palo Alto, CA), Geiser; Timothy G. (La Honda, CA), Hunkapiller; Michael W. (San Carlos, CA), Kent; Stephen B. H. (Pasadena, CA), Marriott; Mark P. (Los Altos, CA), Ramstad; Paul O. (Oakland, CA), Nordman; Eric S. (San Bruno, CA) |
Assignee: |
Applied Biosystems, Inc.
(Foster City,
CA)
|
Appl. No.: | 06/592,638 |
Filed: | March 23, 1984 |
Current U.S. Class: | 422/62 ; 422/107; 422/109; 422/111; 422/116; 422/134; 422/138; 435/317.1; 700/268 |
Current International Class: | B01J 19/00 (20060101); C07K 1/00 (20060101); C07K 1/04 (20060101); C07K 001/08 () |
Field of Search: | 422/62,107,109,110,111,116,129,134,138 260/112R 435/69,71,289,290,317 536/27 364/500 935/88 |
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