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United States Patent | 4,322,499 |
Baxter , et al. | March 30, 1982 |
A technique suitable for cloning a cDNA having a base sequence coding for the ACTH/LPH precursor is disclosed. The invention is exemplified by the cloning of a cDNA fragment comprising a base sequence coding for the endorphin region. The fragment, hereinafter termed the endorphin gene cDNA sequence, was obtained from cultured mouse pituitary tumor cells known to produce the ACTH/LPH precursor protein.
Current U.S. Class: | 435/252.33 ; 435/252.3; 435/320.1; 435/69.1; 435/69.4; 435/91.41; 536/23.51; 930/70; 930/80 |
Current International Class: | C07K 14/435 (20060101); C07K 14/665 (20060101); C12N 001/00 () |
Field of Search: | 435/172,253,317,255 |
4237224 | December 1980 | Cohen et al. |
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