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United States Patent | 4,158,049 |
Pelley , et al. | June 12, 1979 |
Schistosoma mansoni eggs are ground to liberate soluble egg antigens which are fractionated and purified by absorption, gel filtration and/or ion exchange chromatography, and then radioactively labelled with .sup.125 I. A fraction, identified as MSA.sub.1, is specific to the mature egg stage of Schistosoma mansoni and thus can be used to detect the presence of antibody to mature Schistosoma mansoni eggs in a patient's serum even as compared with mature eggs of different Schistosoma (species specific) or of Schistosoma mansoni worms but not mature eggs (stage specific).
Inventors: | Pelley; Ronald P. (Cleveland, OH), Warren; Kenneth S. (Cleveland Heights, OH) |
Assignee: |
Edna McConnell Clark Foundation
(New York,
NY)
|
Appl. No.: | 05/727,205 |
Filed: | September 27, 1976 |
Current U.S. Class: | 436/542 ; 435/7.22; 436/543; 436/545; 436/811 |
Current International Class: | A61K 39/00 (20060101); G01N 33/53 (20060101); G01N 033/16 (); A61K 039/00 (); A61K 043/00 () |
Field of Search: | 424/1,1.5,9,88 195/103.5 |
3860483 | January 1975 | Senft |
Boros et al., Journal of Experimental Medicine, vol. 132, No. 3, Sep. 1, 1970, pp. 488-507. . Colley, The Journal of Immunology, vol. 115, No. 1, Jul., 1975, pp. 150-156. . Boros, et al., The Journal of Immunology, vol. 114, No. 5, May 1975, pp. 1437-1441. . Pelley, et al., Infection and Immunity, vol. 13, No. 4, Apr. 1976, pp. 1176-1183.. |