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United States Patent 7,491,690
Stupp ,   et al. February 17, 2009

Self-assembly and mineralization of peptide-amphiphile nanofibers

Abstract

Peptide-amphiphilic compositions capable of self-assembly into useful nanostructures.


Inventors: Stupp; Samuel I. (Chicago, IL), Hartgerink; Jeffrey D. (Pearland, TX), Beniash; Elia (Auburndale, MA)
Assignee: Northwestern University (Evanston, IL)
Appl. No.: 10/294,114
Filed: November 14, 2002

Related U.S. Patent Documents

Application NumberFiling DatePatent NumberIssue Date
60333074Nov., 2001

Current U.S. Class: 514/1.1 ; 514/3.2; 530/327; 530/329
Current International Class: A61K 38/08 (20060101); C07K 7/06 (20060101); C07K 7/08 (20060101)
Field of Search: 514/2,12-19,762,788.1,944


References Cited [Referenced By]

U.S. Patent Documents
4554101 November 1985 Hopp
4930077 May 1990 Fan
5130123 July 1992 Reynolds et al.
5208111 May 1993 Decher et al.
5670483 September 1997 Zhang et al.
5733868 March 1998 Peterson et al.
5843780 December 1998 Thomson
5871767 February 1999 Dionne et al.
5955343 September 1999 Holmes et al.
5993541 November 1999 Litvin et al.
6051272 April 2000 Stupp et al.
6085206 July 2000 Domini et al.
6096863 August 2000 Fields et al.
6156321 December 2000 Thorpe et al.
6181909 January 2001 Burstein et al.
6201065 March 2001 Pathak et al.
6265539 July 2001 Arlinghaus
6269368 July 2001 Diamond
6270765 August 2001 Deo et al.
6391297 May 2002 Halvorsen
6458924 October 2002 Knudsen et al.
6473730 October 2002 McKeown et al.
6548048 April 2003 Cuthbertson et al.
6548630 April 2003 Zhang et al.
6562619 May 2003 Gearhart et al.
6800481 October 2004 Holmes et al.
6855329 February 2005 Shakesheff et al.
6890654 May 2005 Stupp et al.
7371719 May 2008 Stupp et al.
7390526 June 2008 Stupp et al.
2002/0007217 January 2002 Jacob et al.
2002/0046018 April 2002 Marcu et al.
2002/0142277 October 2002 Burstein et al.
2002/0160471 October 2002 Kisiday et al.
2003/0059906 March 2003 Hubbell et al.
2003/0092672 May 2003 Darcy et al.
2003/0176335 September 2003 Zhang et al.
2004/0001893 January 2004 Stupp et al.
2004/0018961 January 2004 Stupp et al.
2004/0022718 February 2004 Stupp et al.
2004/0258726 December 2004 Stupp et al.
2005/0208589 September 2005 Stupp et al.
2005/0209145 September 2005 Stupp et al.
2005/0214257 September 2005 Zhao et al.
2005/0272662 December 2005 Stupp et al.
2006/0149036 July 2006 Stupp et al.
2006/0247165 November 2006 Stupp et al.
Foreign Patent Documents
03099096 Apr., 1991 JP
WO 97/14713 Apr., 1997 WO
WO 97/20639 Jun., 1997 WO
WO 98/07752 Feb., 1998 WO
99/36107 Jul., 1999 WO
99/55383 Nov., 1999 WO
WO 00/13710 Mar., 2000 WO
00/45831 Aug., 2000 WO
WO 0044808 Aug., 2000 WO
WO 00/52145 Sep., 2000 WO
WO 00/64481 Nov., 2000 WO
WO 01/00650 Jan., 2001 WO
WO 02/062969 Aug., 2002 WO
WO 03/040336 May., 2003 WO
WO 03/054146 Jul., 2003 WO
WO 03/070749 Aug., 2003 WO
WO 03/084980 Oct., 2003 WO
WO 03/090255 Oct., 2003 WO
WO 2004/003561 Jan., 2004 WO
WO 2004/018628 Mar., 2004 WO
WO 2004/024778 Mar., 2004 WO
WO 2004/046167 Jun., 2004 WO
WO 2004/072104 Aug., 2004 WO
2004/091370 Oct., 2004 WO
WO 2004/106359 Dec., 2004 WO
WO 2005/003292 Jan., 2005 WO
WO 2005/056039 Jun., 2005 WO
WO 2005/056576 Jun., 2005 WO
WO 2006/096614 Sep., 2006 WO

Other References

Yang et al. Physiochemical aspects of drug delivery and release from poly-based colloids,: Current Opinion in Colloid & Interface Science (Mar. 2000) 5(1,2): 132-143. cited by examiner .
Fernandez et al. Synthesis and Physiochemical Characterization of Cyclic Laminin Related Peptdies. Langmuir. 1998. vol. 14, pp. 3625-3630. cited by examiner .
Nomizu et al. The all-D-configuration segment containing the IKVAV sequence of laminin A chain has similar activities to the all-L-peptide in vitro and in vivo. J. Biol. Chem. 1992 267: 14118-14121. cited by examiner .
Shimizu, T., Hato, M. Self-assembling properties of synthetic peptidic lipids. Biochimica et Biophysica Acta, 1147 (1993) 50-58. cited by other .
Yu, Y-C., Berndt, P., Tirrell, M., and Fields, G.B. Self-assembling amphiphiles for construction of protein molecular architecture. J. Am. Chem. Soc. 1996, 118, 12515-12520. cited by other .
Hartgerink, J. D., Beniash, El. and Stupp, S.I. Peptide-amphiphile nanofibers: A versatile scaffold for the preparation of self-assembling materials. PNAS, 2002, 99(8):5133-5138. cited by other .
Hartgerink, J.D., Beniash, E., and Stupp, S.I. Self-assembly and mineralization of peptide-amphiphile nanofibers. Science, Nov. 23, 2001, vol. 294(5547):1684-1688. cited by other .
Nomizu, Motoyoshi, Keizo Yamamura, Hynda K. Kleinman, and Yoshihiko Yamada. Aug. 1, 1993. "Multimeric Forms of Tyr-Ile-Gly-Ser-Arg (YIGSR) Peptide Enhance the Inhibtion of Tumor Growth and Metastasis." Cancer Research. vol. 53, pp. 3459-3461. cited by other .
Jin, Young-Gu and K. J. Chang. Feb. 26, 2001. "Mechanism for the Enhanced Diffusion of Charged Oxygen Ions in SiO2." Physical Review Letters. vol. 86, No. 9, pp. 1793-1796. cited by other .
Matsui, Hiroshi and Robert MacCuspie. Dec. 2001. "Metalloporphyrin Nanotube Fabrication Using Peptide Nanotubes as Templates." Nano Letters. vol. 1, No. 12, pp. 671-675. cited by other .
Irvine, Darrell J. and Anne M. Mayes. 2001. "Nanoscale Clustering of RGD Peptides at Surfaces Using Comb Polymers. 1. Synthesis and Characterization of Comb Thin Films." Biomacromolecules. vol. 2, No. 1, pp. 85-94. cited by other .
Matsui, Hiroshi, Precila Porrata, Gary E. Douberty, Jr. 2001. "Protein Tubule Immobilization on Self-Assembled Monolayers on Au Substrates." Nano Letters. vol. 1, No. 9, pp. 461-464. cited by other .
Slocik, Joseph M., Joshua T. Moore, and David W. Wright. Mar. 2002. Monoclonal Antibody Recognition of Histidine-Rich Peptide Encapsulated Nanoclusters. Nano Letters. vol. 2, No. 3, pp. 169-173. cited by other .
Shih, Sheng-Ming, Wei-Fang Su, Yuh-Jiuan Lin, Cen-Shawn Wu, and Chii-Dong Chen. 2002. "Two-Dimensional Arrays of Self-Assembled Gold and Sulfur-Containing Fullerene Nanoparticles." Langmuir. vol. 18, No. 8, pp. 3332-3335. cited by other .
Wong, Michael S., Jennifer N. Cha, Kyoung-Shin Choi, Timothy J. Deming, and Galen D. Stucky. 2002. "Assembly of Nanoparticles into Hollow Spheres Using Block Copolypeptides." Nano Letters. vol. 2, No. 6, pp. 583-587. cited by other .
"AccessScience Search Results. Amphiphile." Accessed online May 7, 2007. http://www.accessscience.com/search/asearch? location=titlestext&newSearch=1&pubpriv=private&categories=dictionary&cat- egval=dictionary&text=amphiphile. McGraw-Hill Encyclopedia of Science & Technology Online. cited by other .
Margomenou-Leonidopoulou, G. 1994. "Thermotropic Mesophases of Ionic Amphiphiles II. Ionic Amphiphiles in Aqueous Media." Journal of Thermal Analysis. vol. 42, pp. 1041-1061. cited by other .
Rappolt, Michael and Gert Rapp. 1996. "Structure of the Stable and Metastable Ripple Phase of Dipalmitoylphosphatidylcholine." Eur. Biophys. J. vol. 24, pp. 381-386. cited by other .
Goveas, J. L. and S. T. Milner. 1997. "Dynamics of the Lamellar--Cylindrical Transition in Weakly Segregated Diblock Copolymer Melts." Macromolecules. vol. 30, No. 9, pp. 2605-2612. cited by other .
Munson, John B. and Stephen B. McMahon. 1997. "Effects of GDNF on Axotomized Sensory and Motor Neurons in Adult Rats." European Journal of Neuroscience. vol. 9, pp. 1126-1129. cited by other .
Yagi, Nobuhiro, Yoshikatsu Ogawa, Masato Kodaka, Tomoko, Okada, Takenori Tomohiro, Takeo Konakahara, and Hiroaki Okuno. 1999. "A Surface-Modified Functional Liposome Capable of Binding to Cell Membranes." Chem. Commun. pp. 1687-1688. cited by other .
Luo, Yi and Glenn D. Prestwich. 2001. "Novel Biomaterials for Drug Delivery." Expert Opin. Ther. Patents. vol. 11, No. 9, pp. 1395-1410. cited by other .
Marchi-Artzner, Valerie, Barbara Lorz, Ulrike Hellerer, Martin Kantlehner, Horst Kessler, and Erich Sackmann. 2001. "Selective Adhesion of Endothelial Cells to Artificial Membranes with a Synthetic RGD-Lipopeptide." Chem. Eur. J. vol. 7, No. 5, pp. 1095-1101. cited by other .
Blight, Andrew R. Nov. 2002. "Miracles and Molecules--Progress in Spinal Cord Repair." Nature Neuroscience Supplement. vol. 5, pp. 1051-1054. cited by other .
Rodger, Allison, Jascindra Rajendra, Rachel Marrington, Malin Ardhammar, Bengt Norden, Jonathan D. Hirst, Andrew T. B. Gilbert, Timothy R. Dafforn, David J. Halsalt, Cheryl A. Woolhead, Colin Robinson, Teresa J. T. Pinheiro, Jurate Kazlauskaite, Mark Seymour, Niuvis Perez, and Michael J. Hannon. 2002. "Flow Oriented Linear Dichroism to Probe Protein Orientation in Membrane Environments." Phys. Chem. Chem. Phys. vol. 4, pp. 4051-4057. cited by other .
Silva, G. A., K. L. Kehl, K. L. Niece, and S. I. Stupp. May 4, 2003. "Nanoengineered Peptide Amphiphile Network for Photoreceptor Replacement in Degenerative Retinal Disorders." Investigative Ophthalmology & Visual Science. Abstract No. 492 from Annual Meeting of the Association for Research in Vision and Opthalmology. cited by other .
Brandenburg, Klaus, Frauke Wagner, Mareike Muller, Holger Heine, Jorg Andra, Michel H. J. Koch, Ulrich Zahringer, and Ulrich Seydel. 2003. "Physicochemical Characterization and Biological Activity of a Glycoglycerolipid from Mycoplasma fermentans." Eur. J. Biochem. vol. 270, pp. 3271-3279. cited by other .
Czeisler, C., V. M. Tysseling-Mattiace, G. A. Silva, S. I. Stupp, and J. A. Kessler. 2003. "Behavoral Improvement and Increased Survival Rate after Treatment with a Self Assembling Gel in a Rat Model of Spinal Cord Injury." 2003 Abstract Viewer/Itinerary Planner. Program No. 245.22. Washington, DC: Society for Neuroscience. Printed Feb. 5, 2007. Page 1. http://sfn.scholarone.com/itin2003/main.html?new.sub.-- page.sub.--id=126&abstract.sub.--id= 1554... cited by other .
Schmidt, Christine E. and Jennie Baier Leach. 2003. "Neural Tissue Engineering: Strategies for Repair and Regeneration." Annu. Rev. Biomed. Eng. vol. 5, pp. 293-347. cited by other .
t' Hart, Bert A. and Sandra Amor. 2003. "The Use of Animal Models to Investigate the Pathogenesis of Neuroinflammatory Disorders of the Central Nervous System." Current Opinion in Neurology. vol. 16, pp. 375-383. cited by other .
Benlash, Elia, Jeffery D. Hartgerink, Hannah Storrie, John C. Stendahl, and Samuel I. Stupp. 2005. "Self-Assembling Peptide Amphiphile Nanofiber Matrices for Cell Entrapment." Acta Biomaterialia. vol. 1, pp. 387-397. cited by other .
Hoke, Ahmet, Aug. 2006. "Mechanisms of Disease: What Factors Limit the Success of peripheral Nerve Regeneration in Humans?" Nature Clinical Practice Neurology. vol. 2, No. 8, pp. 448-454. cited by other .
Kokkoli, Efrosini, Anastasia Mardilovich, Alison Wedekind, Emilie l. Rexeisen, Ashish Garg, and Jennifer A. Craig. 2006. "Self-Assembly and Applications of Biomimetic and Bioactive Peptide-Amphiphiles." Soft Matter. vol. 2, pp. 1015-1024. cited by other .
The LabRat.com. 2007, updated. Hank's Buffered Salt Solution (HBSS) Recipe. http://www.thelabrat.com/protocolsHanks.shtml. Printed Jan. 19, 2007. pp. 1-2. cited by other .
Copping, Aaron M. and Vanda R. G. Pond. Dec. 9, 1950. "Folic Acid as a Growth-Factor for the Rat." Nature. No. 4232, p. 993. cited by other .
Mulloy, Barbara and Mark J. Forster. 2000. "Conformation and Dynamics of Heparin and Heparan Sulfate." Glycobiology. vol. 10, No. 11, pp. 1147-1156. cited by other .
Bruggeman, Holger, Sebastian Baumer, Wolfgang Florian Fricke, Amim Wiezer, Heiko Liesegang, Iwona Decker, Christina Herzberg, Rosa Martinez-Arias, Rainer Merkl, Anke Henne, and Gerhard Gottschalk. Feb. 4, 2003. "The Genome Sequence of Clostridium tetani, the Causative Agent of Tetanus Disease." PNAS. vol. 100, No. 3, pp. 1316-1321. cited by other .
Invitrogen. Printed Jan. 22, 2008. "Dulbecco's Modified Eagle Medium (D-MEM) (1X) Liquid (High Glucose)." http://www.invitrogen.com/content.cfm?pageId=95&fuseaction=MediaForm.dsp.- sub.--mediaForm&productId... cited by other .
Uniprot entry for Q899Z6. Printed Mar. 14, 2008. http://www.pir.uniprot.org/cgi-bin/upEntry?id=Q899Z6.sub.--CLOTE. 3 pages. cited by other .
Jackowski, Andre. 1995. "Neural Injury Repair: Hope for the Future as Barriers to Effective CNS Regeneration Become Clearer." J. Neurosurg. vol. 9, pp. 303-317. cited by other .
Kanke, Rene, Amir W. Fahmi, Syed A. M. Tofail, Jason Clohessy, Miroslav Mihov, and Vincent J. Cunnane. 2005. "Electrochemical Nucleation of Gold Nanoparticles in a Polymer Film at a Liquid-Liquid Interface." Langmuir. vol. 21, No. 3, pp. 1001-1008. cited by other .
Kibbey, Maura C., Mathias Jucker, Benjamin S. Weeks, Rachael L. Neve, William E. Van Nostrand, and Hynda K. Kleinman. Nov. 1993. ".beta.-Amyloid Precursor Protein Binds to the Neurite-Promoting IKVAV Site of Laminin." Proc. Natl. Acad. Sci. U.S.A. vol. 90, pp. 10150-10153. cited by other .
Oka, Kazunari, Masaaki Yamamoto, Toshiharu Nonaka, and Masamichi Tomonaga. Apr. 1996. "The Significance of Artificial Cerebrospinal Fluid as Perfusate and Endoneurosurgery." Neurosurgery Online. vol. 38, No. 4, pp. 733-736. cited by other .
Rapaport, Hanna, Kristian Kjaer, Torben R. Jensen, Leslie Leiserowitz, and David A. Tirrell. 2000. "Two-Dimensional Order in .beta.-Sheet Peptide Monolayers." Journal of the American Chemical Society. vol. 122, No. 50, pp. 12523-12529. cited by other .
Avrahami, Dorit and Yechiel Shai. 2002. "Conjugation of a Magainin Analogue with Lipophilic Acids Controls Hydrophobicity, Solution Assembly, and Cell Selectivity." Biochemistry. vol. 41, No. 7, pp. 2254-2263. cited by other .
Yamada, Masanori, Yuichi Kadoya, Shingo Kasai, Kozue Kato, Mayumi Mochizuki, Norio Nishi, Nobuhisa Watanabe, Hynda K. Kleinman, Yoshihiko Yamada, and Motoyoshi Nomizu. 2002. "Ile-Lys-Val-Ala-Val (IKVAV)-Containing Laminin .alpha.1 Chain Peptides From Amyloid-like Fibrils." FEBS Letters. vol. 530, pp. 48-52. cited by other .
McGregor,Clare-Louise, Lu Chen, Neil C. Pomroy, Peter Hwang, Sandy Go, Avijit Chakrabartty, and Gilbert G. Prive. Feb. 2003. "Lipopeptide Detergents Designed for the Structural Study of Membrane Proteins." Nature Biotechnology. vol. 21, pp. 171-176. cited by other .
Ohmori, Hideya, Yasumitsu Sato, and Akiyoshi Namiki. 2004. "The Anticonvulsant Action of Propofol on Epileptiform Activity in Rat Hippocampal Slices." Anesth. Analg. vol. 99, pp. 1095-1101. cited by other .
Shahraki, Ali and Trevor W. Stone. 2004. "Blockade of Presynaptic Adenosine A1 Receptor Responses by Nitric Oxide and Superoxide in Rat Hippocampus." European Journal of Neuroscience. vol. 20, pp. 719-728. cited by other .
Sone, Eli D. and Samuel I. Stupp. 2004. "Semiconductor-Encapsulated Peptide-Amphiphile Nanofibers." Journal of the American Chemical Society. vol. 126, No. 40, pp. 12756-12757. cited by other .
Smith, L. A. and P. X. Ma. 2004. "Nano-Fibrous Scaffolds for Tissue Engineering." Colloids and Surfaces. B: Biointerfaces. vol. 39, pp. 125-131. cited by other .
Tsonchev, Stefan, George C. Schatz, and Mark A. Ratner. 2004. "Electrostatically-Directed Self-Assembly of Cylindrical Peptide Amphiphile Nanostructures." J. Phys. Chem. B. vol. 108, No. 26, pp. 8817-8822. cited by other .
Tsonchez, Stefan, Alessandro Troisi, George C. Schatz, and Mark A. Ratner. 2004. "All-Atom Numerical Studies of Self-Assembly of Zwitterionic Peptide Amphiphiles." J. Phys. Chem. B. vol. 108, No. 39, pp. 15278-15284. cited by other .
Tsonchev, Stefan, Alessandro Troisi, George C. Schatz, and Mark A. Ratner. 2004. "On the Structure and Stability of Self-Assembled Zwitterionic Peptide Amphiphiles: A Theoretical Study." Nano Letters. vol. 4, No. 3, pp. 427-431. cited by other .
Arnold, Michael S., Mustafa O. Guler, Mark C. Hersam, and Samuel I. Stupp. 2005. "Encapsulation of Carbon Nanotubes by Self-Assembling Peptide Amphiphiles." Langmuir. vol. 21, No. 10, pp. 4705-4709. cited by other .
Behanna, Heather A., Jack J. J. M. Donners, Alex C. Gordon, and Samuel I. Stupp. 2005. "Coassembly of Amphiphiles with Opposite Peptide Polarities into Nanofibers." Journal of the American Chemical Society. vol. 127, No. 4, pp. 1193-1200. cited by other .
Bitton, Ronit, Judith Schmidt, Markus Biesalski, Raymond Tu, Matthew Tirrell, and Havazelet Bianco-Peled. 2005. "Self-Assembly of Model DNA-Binding Peptide Amphiphiles." Langmuir. vol. 21, No. 25, pp. 11888-11895. cited by other .
Bull, Steve R., Mustafa O. Guler, Rafael E. Bras, Palamadai N. Venkatasubramanian, Samuel I. Stupp, and Thomas J. Meade. 2005. "Magnetic Resonance Imaging of Self-Assembled Biomaterial Scaffolds." Bioconjugate Chem. vol. 16, No. 6, pp. 1343-1348. cited by other .
de Loos, Maaike, Ben L. Feringa, and Jan H. van Esch. 2005. "Design and Application of Self-Assembled Low Molecular Weight Hydrogels." Eur. J. Org. Chem. pp. 3615-3631. cited by other .
Guler, Mustafa O., Randal C. Claussen, and Samuel I. Stupp. 2005. "Encapsulation of Pyrene Within Self-Assembled Peptide Amphiphile Nanofibers." Journal of Materials Chemistry. vol. 15, pp. 4507-4512. cited by other .
Guler, Mustafa O., Jonathan K. Pokorski, Daniel H. Appella, and Samuel I. Supp. 2005. "Enhanced Oligonucleotide Binding to Self-Assembled Nanofibers." Bioconjugate Chem. vol. 16, No. 3, pp. 501-503. cited by other .
Jun, Ho-Wook, Virany Yuwono, Sergey E. Paramonov, and Jeffrey D. Hartgerink. 2005. "Enzyme-Mediated Degradation of Peptide-Amphiphile Nanofiber Networks." Adv. Mater. vol. 17, pp. 2612-2617. cited by other .
Silva, Gabriel A. 2005. "Nanotechnology Approaches for the Regeneration and Neuroprotection of the Central Nervous System." Surgical Neurology. vol. 63, pp. 301-306. cited by other .
Silva, Gabriel A. 2005. "Small Neuroscience: The Nanostructure of the Central Nervous System and Emerging Nanotechnology Applications." Current Nanoscience. vol. 1, No. 3, pp. 225-236. cited by other .
Solis., F. J., S. I. Stupp, and M. Olvera de la Cruz. 2005. "Charge Induced Pattern Formation on Surfaces: Segregation in Cylindrical Micelles of Cationic-Anionic Peptide-Amphiphiles." The Journal of Chemical Physics. vol. 122, No. 5, 054905-1-054905-9. cited by other .
Tovar, John D., Randal C. Claussen, and Samuel I. Stupp. 2005. "Probing the Interior of Peptide Amphiphile Supramolecular Aggregates." Journal of the American Chemical Society. vol. 127, No. 20, pp. 7337-7345. cited by other .
Hosseinkhani, Hossein, Mohsen Hosseinkhani, and Hisatoshi Kobayoshi. Jul. 2006. "Design of Tissue-Engineered Nanoscaffold Through Self-Assembly of Peptide Amphiphile." Journal of Bioactive and Compatible Polymers. vol. 21, No. 4, pp. 277-296. cited by other .
Engler, Adam J., Shamik Sen, H. Lee Sweeney, and Dennis E. Discher. Aug. 25, 2006. "Matrix Elasticity Directs Stem Cell Lineage Specification." Cell. vol. 126, pp. 677-689. cited by other .
Brunsveld, L., J. Kuhlmann, and H. Waldmann. 2006. "Synthesis of Palmitoylated Ras-Peptides and -Proteins." Methods. vol. 40, pp. 151-165. cited by other .
Elgersma, Ronald C., Tania Meijneke, Remco de Jong, Arwin J. Brouwer, George Posthuma, Dirk T. S. Rijkers, and Rob M. J. Liskamp. 2006. "Synthesis and Structural Investigations of N-alkylated .beta.-peptidosulfonamide-peptide Hybrids of the Amyloidogenic Amylin(20-29) Sequence: Implications of Supramolecular Folding for the Design of Peptide-Based Bionanomaterials." Organic & Biomolecular Chemistry. vol. 4, pp. 3587-3597. cited by other .
Guler, Mustafa O., Lorraine Hsu, Stephen Soukasene, Daniel A. Harrington, James F. Hulvat, and Samuel I. Stupp. 2006. "Presentation of RGDS Epitopes on Self-Assembled Nanofibers of Branched Peptide Amphiphiles." Biomacromolecules. vol. 7, No. 6, pp. 1855-1863. cited by other .
Harrington, Daniel A., Earl Y. Cheng, Mustafa O. Guler, Leslie K. Lee, Jena L. Donovan, Randal C. Claussen, and Samuel I. Stupp. 2006. "Branched Peptide-Amphiphiles as Self-Assembling Coatings for Tissue Engineering Scaffolds." Journal of Biomedical Materials Research Part A. pp. 157-167. cited by other .
Hosseinkhani, Hossein, Mohsen Hosseinkhani, Ali Khademhosseini, Hisatoshi Kobayashi, and Yasuhiko Tabata. 2006. "Enhanced Angiogenesis Through Controlled Release of Basic Fibroblast Growth Factor from Peptide Amphiphile for Tissue Regeneration." Biomaterials. vol. 27, pp. 5836-5844. cited by other .
Mardilovich, Anastasia, Jennifer A. Craig, Matthew Q. McCammon, Ashish Garg, and Efrosini Kokkoli. 2006. "Design of a Novel Fibronectin-Mimetic Peptide-Amphiphile for Functionalized Biomaterials." Langmuir. vol. 22, No. 7, pp. 3259-3264. cited by other .
Paramonov, Sergey E., Ho-Wook Jun, and Jeffrey D. Hartgerink. 2006. "Self-Assembly of Peptide-Amphiphile Nanofibers: The Roles of Hydrogen Bonding and Amphiphilic Packing." Journal of the American Chemical Society. vol. 128, No. 22, pp. 7291-7298. cited by other .
Rajangam, Kanya, Heather A. Behanna, Michael J. Hui, Xiaoqiang Han, James F. Hulvat, Jon W. Lomasney, and Samuel I. Stupp. 2006. "Heparin Binding Nanostructures to Promote Growth of Blood Vessels." Nano Letters. vol. 6, No. 9, pp. 2086-2090. cited by other .
Reches, Meital and Ehud Gazit. 2006. "Molecular Self-Assembly of Peptide Nanostructures: Mechanism of Association and Potential Uses." Current Nanoscience. vol. 2, No. 2, pp. 105-111. cited by other .
Stendahl, John C., Mukti S. Rao, Mustafa O. Guler, and Samuel I. Stupp. 2006. "Intermolecular Forces in the Self-Assembly of Peptide Amphiphile Nanofibers." Advanced Functional Materials. vol. 16, pp. 499-508. cited by other .
Behanna, Heather A., Kanya Rajangam, and Samuel I. Stupp. 2007. "Modulation of Fluoresence Through Coassembly of Molecules in Organic Nanostructures." Journal of the American Chemical Society. vol. 129, No. 2, pp. 321-327. cited by other .
Meijer, Joris T., Marjolijn Roeters, Valentina Viola, Dennis W. P. M. Lowik, Gert Vriend, and Jan C. M. van Hest. 2007. "Stabilization of Peptide Fibrils Hydrophobic Interaction." vol. 23, No. 4, pp. 2058-2063. cited by other .
U.S. Appl. No. 11/337,316, filed Jan. 23, 2006, Stupp et al. cited by other .
Brown, Walter E. Dec. 15, 1962. "Octacalcium Phosphate and Hydroxyapatite." Nature. vol. 196, pp. 1048-1050. cited by other .
Liang, W. Y. and A. D. Yoffe. Jan. 8, 1968. "Transmission Spectra of ZnO Single Crystals." Physical Review Letters. vol. 20, No. 2, pp. 59-62. cited by other .
Greenfield, Norma and Gerald D. Fasman. Oct. 1969. "Computed Circular Dichroism Spectra for the Evaluation of Protein Conformation." Biochemistry. vol. 8, No. 10, pp. 4108-4116. cited by other .
Hantke, Klaus and Volkamr Braun. 1973. "Covalent Binding of Lipid to Protein: Diglyceride and Amide-Linked Fatty Acid at the N-Terminal End of the Murein-Lipoprotein of the Escherichia coli Outer Membrane." Eur. J. Biochem. vol. 34, No. 2, pp. 284-296. cited by other .
Balcerski, James S., E. S. Pysh, G. M. Bonora, and C. Toniolo. Jun. 9, 1976. "Vacuum Ultraviolet Circular Dichroism of .beta.-Forming Alkyl Oligopeptides." Journal of the American Chemical Society, vol. 98, No. 12, pp. 3470-3473. cited by other .
Jacobson, Bruce S. and Daniel Branton. Jan. 21, 1977. "Plasma Membrane: Rapid Isolation and Exposure of the Cytoplasmic Surface by Use of Positively Charged Beads." Science. vol. 195, No. 4275, pp. 302-304. cited by other .
Biesecker, G., J. Ieuan Harris, J. C. Thierry, J. E. Walker, and A. J. Wonacott. Mar. 24, 1977. Nature. vol. 266, pp. 328-333. cited by other .
Kelly, Margaret M., E. S. Pysh, G. M. Bonora, and C. Toniolo. May 11, 1977. "Vacuum Ultraviolet Circular Dichroism of Protected Homooligomers Derived from L-Leucine." Journal of the American Chemical Society. vol. 99, No. 10, pp. 3264-3266. cited by other .
Blumenthal, N. C., A. S. Posner, L. D. Silverman, and L. C. Rosenberg. 1979. "Effect of Proteoglycans on in Vitro Hydroxyapatite Formation." Calcified Tissue International. vol. 27, No. 1, pp. 75-82. cited by other .
Richardson, P. M., U. M. McGuinness, and A. J. Aguayo. Mar. 20, 1980. "Axons from CNS Neurones Regenerate into PNS Grafts." Nature. vol. 284, pp. 264-265. cited by other .
Lim, Franklin and Anthony M. Sun. Nov. 21, 1980. "Microencapsulated Islets as Bioartificial Endocrine Pancreas." Science. vol. 210, No. 4472, pp. 908-910. cited by other .
Jain, Rakesh K., Chhitar M. Gupta, and Nitya Anand. 1981. "Synthesis of Peptidylglycophospholipids, Novel Derivatives of Muranyl-Dipeptide." Tetrahedron Letters. vol. 22, No. 24, pp. 2317-2320. cited by other .
Sarin, Virender K., Stephen B. H. Kent, James P. Tam, and R. B. Merrifield. 1981. "Quantitative Monitoring of Solid-Phase Peptide Synthesis by the Ninhydrin Reaction." Analytical Biochemistry. vol. 117, pp. 147-157. cited by other .
Yannas, I. V., J. F. Burke, D. P. Orgill, E. M. Skrabut. Jan. 8, 1982. "Wound Tissue Can Utilize a Polymeric Template to Synthesize a Functional Extension of Skin." Science. vol. 215, No. 4529, pp. 174-176. cited by other .
Montesano, R. L. Orci, and P. Vassalli. Nov. 1983. "In Vitro Rapid Organization of Endothelial Cells into Capillary-like Networks is Promoted by Collagen Matrices." The Journal of Cell Biology. vol. 97, pp. 1648-1652. cited by other .
Pierschbacher, Michael D. and Erkki Ruoslahti. May 3, 1984. "Cell Attachment Activity of Fibronectin Can Be Duplicated by Small Synthetic Fragments of the Molecule." Nature. vol. 309, pp. 30-33. cited by other .
Landis, W. J. and J. R. Martin. Apr.-Jun. 1984. "X-Ray Photoelectron Spectroscopy Applied to Gold-Decorated Mineral Standards of Biological Interest." J. Vac. Sci. Technol. vol. A 2, No. 2, pp. 1108-1111. cited by other .
Thompson, Nancy L., Adrienne A. Brian, and Harden M. McConnell. 1984. "Covalent Linkage of a Synthetic Peptide to a Fluorescent Phospholipid and Its Incorporation into Supported Phospholipid Monolayers." Biochimica et Biophysica Acta. vol. 772, pp. 10-19. cited by other .
Yamada, Kimiho, Hirotaka Ihara, Toshio Ide, Takanori Fukumoto, and Chuichi Hirayama. 1984. "Formation of Helical Super Structure from Single-Walled Bilayers by Amphiphiles with Oligo-L- Glutamic Acid-Head Group." Chemistry Letters. No. 10, pp. 1713-1716. cited by other .
Addadi, L. and S. Weiner. Jun. 15, 1985. "Interactions Between Acidic Proteins and Crystals: Stereochemical Requirements in Biomineralization." Proceedings of the National Academy of Sciences of the United States of America. vol. 82, No. 12, pp. 4110-4114. cited by other .
"Public Health Service Policy on Humane Care and Use of Laboratory Animals." Sep. 1986. Office for Protection from Research Risks (OPRR), National Institutes of Health. cited by other .
Weiner, Stephen and Wolfie Traub. Oct. 1986. "Organization of Hydroxyapatite Crystals Within Collagen Fibrils." FEBS Letters. vol. 206, No. 2, pp. 262-266. cited by other .
Mann, Stephen, John P. Hannington, and R. J. P. Williams. Dec, 11, 1986. "Phospholipid Vesicles as a Model System for Biomineralization." Nature. vol. 324, pp. 565-567. cited by other .
Krimm, Samuel and Jagdeesh Bandekar. 1986. "Vibrational Spectroscopy and Conformation of Peptides, Polypeptides, and Proteins." Advances in Protein Chemistry, vol. 38, pp. 181-364. cited by other .
de Groot, K., R. Geesink, C. P. A. T. Klein, and P. Serekian. Dec. 1987. "Plasma Sprayed Coatings of Hydroxylapatite." Journal of Biomedical Materials Research. vol. 21, No. 12, pp. 1375-1381. cited by other .
Bresnahan, J. C., M. S. Beattie, F. D. Todd III, and D. H. Noyes. 1987. "A Behavorial and Anatomical Analysis of Spinal Cord Injury Produced by a Feedback-Controlled Impaction Device." Experimental Neurology. vol. 95, pp. 548-570. cited by other .
Moscatelli, David. 1987. "High and Low Affinity Binding Sites for Basic Fibroblast Growth Factor on Cultured Cells: Absence of a Role for Low Affinity Binding in the Stimulation of Plasminogen Activator Production by Bovine Capillary Endothelial Cells." Journal of Cellular Physiology. vol. 131, pp. 123-130. cited by other .
Lambert, Joseph B., Herbert F. Shurvell, David A. Lightner, and R. Graham Cooks. 1987. "Group Frequencies: Infrared and Raman." Introduction to Organic Spectroscopy. New York: Macmillan Publishing Company. pp. 169-182. cited by other .
Cook, Stephen D., Kevin A. Thomas, John F. Kay, and Michael Jarcho. Jul. 1988. "Hydroxyapatite-Coated Titanium for Orthopedic Implant Applications." Clinical Orthopaedics and Related Research. No. 232, pp. 225-243. cited by other .
Saksela, Olli, David Moscatelli, Andreas Sommer, and Daniel B. Rifkin. Aug. 1988. "Endothelial Cell-Derived Heparan Sulfate Binds Basic Fibroblast Growth Factor and Protects It from Proteolytic Degradation." The Journal of Cell Biology. vol. 107, pp. 743-751. cited by other .
Cardin, Alan D. and H. J. R. Weintraub. Jan./Feb. 1989. "Molecular Modeling of Protein-Glycosaminoglycan Interactions." Arteriosclerosis. vol. 9, No. 1, pp. 21-32. cited by other .
Oonishi, H., M. Yamamoto, H. Ishimaru, E. Tsuji, S. Kuskitani, M. Aono, and Y. Ukon. Mar. 1989. "The Effect of Hydroxyapatite Coating on Bone Growth into Porous Titanium Alloy Implants." The Journal of Bone and Joint Surgery. vol. 71-B, No. 2, pp. 213-216. cited by other .
Friedmann, Theodore. Jun. 16, 1989. "Progress Toward Human Gene Therapy." Science. vol. 244, No. 4910, pp. 1275-1281. cited by other .
Traub, Wolfie, Talmon Arad, and Stephen Wiener. Dec. 15, 1989. "Three-Dimensional Ordered Distribution of Crystals in Turkey Tendon Collagen Fibers." Proceedings of the National Academy of Sciences of the United States of America. vol. 86, No. 24, pp. 9822-9826. cited by other .
Knorr, Reinhard, Arnold Trzeciak, Willi Bannwarth, and Dieter Gillessen. 1989. "New Coupling Reagents in Peptide Chemistry." Tetrahedron Letters. vol. 30, No. 15, pp. 1927-1930. cited by other .
Sambrook, Joseph, Edward F. Fritsch, and Thomas Maniatis. 1989. "Genes Encoding Selectable Markers." Molecular Cloning: A Laboratory Manual. 2.sup.nd New York: Cold Spring Harbor Laboratory Press. pp. 16.9-16.15. cited by other .
Veis, A. 1989. "Biochemical Studies of Vertebrate Tooth Mineralization." Biomineralization. S. Mann, J. Webb, and R. J. P. Williams, editors. Weinheim, Federal Republic of Germany: VCH Verlagsgesellschaft and New York: VCH Publishers. pp. 189-222. cited by other .
Schnell, Lisa and Martin E. Schwab. Jan. 18, 1990. "Axonal Regeneration in the Rat Spinal Cord Produced by an Antibody Against Myelin-Associated Neurite Growth Inhibitors." Nature. vol. 343, pp. 269-272. cited by other .
Ahn, Sang Tae and Thomas A. Mustoe. Jan. 1990. "Effects of Ischemia on Ulcer Wound Healing: A New Model in the Rabbit Ear." Annals of Plastic Surgery. vol. 24, No. 1, pp. 17-23. cited by other .
Van de Pol, Frans C. M. Dec. 1990. "Thin Film ZnO--Properties and Applications." Ceramic Bulletin. vol. 69, No. 12, pp. 1959-1965. cited by other .
Addadi, L., A. Berman, J. Moradian-Oldak, and S. Weiner. Dec. 28, 1990. "Tuning of Crystal Nucleation and Growth by Proteins: Molecular Interactions at Solid-Liquid Interfaces in Biomineralization." Croatica Chemica Acta. vol. 63, No. 3, pp. 539-544. cited by other .
Sukenik, Chaim N., Natarajan Balachander, Lloyd A. Culp, Kristine Lewandowska, and Katherine Merritt. 1990. "Modulation of Cell Adhesion by Modification of Titanium Surfaces with Covalently Attached Self-Assembled Monolayers." Journal of Biomedical Materials Research. vol. 24, pp. 1307-1323. cited by other .
Fields, C. G., D. H. Lloyd, R. L. Macdonald, K. M. Otteson, and R. L. Noble. Mar./Apr. 1991. "HBTU Activation for Automated Fmoc Solid-Phase Peptide Synthesis." Peptide Research. vol. 4, No. 2, pp. 95-101. cited by other .
Murata, Masayuki, Satoshi Kagiwada, Sho Takahashi, and Shun-ichi Ohnishi. Aug. 5, 1991. "Membrane Fusion Induced by Mutual Interaction of the Two Charge-Reversed Amphiphilic Peptides at Neutral pH." The Journal of Biological Chemistry. vol. 56, No. 22, pp. 14353-14358. cited by other .
Harris, Robin, Editor. 1991. Electron Microscopy in Biology: A Practical Approach. New York: Oxford University Press. cited by other .
Jackson, David Y., David S. King, Jean Chmielewski, Sunil Singh, and Peter G. Schultz. 1991. "General Approach to the Synthesis of Short .alpha.-Helical Peptides." Journal of the American Chemical Society. vol. 113, pp. 9391-9392. cited by other .
Polverini, Peter J., Noel P. Bouck, and Farzan Rastinejad. 1991. "Assay and Purification of Naturally Occurring Inhibitor of Angiogenesis." Methods in Enzymology. vol. 198, pp. 440-450. cited by other .
Weiner, Stephen and Wolfie Traub. Feb. 1992. "Bone Structure: From .ANG.ngstroms to Microns." The FASEB Journal. vol. 6, pp. 879-885. cited by other .
Addadi, Lia and Stephen Weiner. 1992. "Control and Design Principles in Biological Mineralization." Angew. Chem. Int. Ed. Engl. vol. 31, pp. 153-169. cited by other .
Beresford, J. N., J. H. Bennett, C. Devlin, P. S. Leboy, and M. E. Owen. 1992. "Evidence for an Inverse Relationship Between the Differentiation of Adipocytic and Osteogenic Cells in Rat Marrow Stromal Cell Cultures." Journal of Cell Science. vol. 102, pp. 341-351. cited by other .
Cook, Stephen D., Kevin A. Thomas, Jeanette E. Dalton, Todd K. Volkman, Thomas S. Whitecloud III, and John F. Kay. 1992. "Hydroxylapatite Coating of Porous Implants Improves Bone Ingrowth and Interface Attachment Strength." Journal of Biomedical Materials Research. vol. 26, pp. 989-1001. cited by other .
Geahlen, Robert L., G. Marc Loudon, Lisa A. Paige, and David Lloyd. 1992. "A General Method for Preparation of Peptides Biotinylated at the Carboxy Terminus." Analytical Biochemistry. vol. 202, pp. 68-70. cited by other .
Ghadiri, M. Reza, Christopher Soares, and Chong Choi. 1992. "Design of an Artificial Four-Helix Bundle Metalloprotein via a Novel Ruthenium(II)-Assisted Self-Assembly Process." Journal of the American Chemical Society. vol. 114, No. 10, pp. 4000-4002. cited by other .
Kunitake, Toyoki. 1992. "Synthetic Bilayer Membranes: Molecular Design, Self-Organization, and Application." Angew. Chem. Int. Ed. Engl. vol. 31, pp. 709-726. cited by other .
Stupp, Samuel I. and Glenn W. Ciegler. 1992. "Organoapatites: Materials for Artificial Bone. I. Synthesis and Microstructure." Journal of Biomedical Materials Research. vol. 26, pp. 169-183. cited by other .
Surewicz, Witold K., Henry H. Mantsch, and Dennis Chapman. Jan. 19, 1993. "Determination of Protein Secondary Structure by Fourier Transform Infrared Spectroscopy: A Critical Assessment." Biochemistry. vol. 32, No. 2, pp. 389-394. cited by other .
Zhang, Shuguang, Todd Holmes, Curtis Lockshin, and Alexander Rich. Apr. 15, 1993. "Spontaneous Assembly of a Self-Complementary Oligopeptide to Form a Stable Macroscopic Membrane." Proceedings of the National Academy of Sciences of the United States of America. vol. 90, No. 8, pp. 3334-3338. cited by other .
Langer, Robert and Joseph P. Vacanti. May 14, 1993. "Tissue Engineering." Science. vol. 260, No. 5110, pp. 920-926. cited by other .
Mulligan, Richard C. May 14, 1993. "The Basic Science of Gene Therapy." Science. vol. 260, No. 5110, pp. 926-932. cited by other .
Massas, R., S. Pitaru, and M. M. Weinreb. Jun. 1993. "The Effects of Titanium and Hydroxyapatite on Osteoblastic Expression and Proliferation in Rat Parietal Bone Cultures." Journal of Dental Research. vol. 72, no. 6, pp. 1005-1008. cited by other .
Archibald, Douglas D. and Stephen Mann. Jul. 29, 1993. "Template Mineralization of Self-Assembled Anisotropic Lipid Microstructures." Nature. vol. 364, pp. 430-433. cited by other .
Atala, Anthony, Linda G. Cima, Wooseob Kim, Keith T. Paige, Joseph P. Vacanti, Alan B. Retik, and Charles A. Vacanti. Aug. 1993. "Injectable Alginate Seeded with Chondrocytes as a Potential Treatment for Vesicoureteral Reflux." The Journal of Urology. vol. 150, No. 2, pp. 745-747. cited by other .
Ross-Murphy, S. B. and K. P. Shatwell. May-Aug. 1993. "Polysaccharide Strong and Weak Gels." Biorheology. vol. 30, Nos. 3 & 4, pp. 217-227. cited by other .
Margalit, Hanah, Nurit Fischer, and Shmuel A. Ben-Sasson. Sep. 15, 1993. "Comparative Analysis of Structurally Defined Heparin Binding Sequences Reveals a Distinct Spatial Distribution of Basic Residues." The Journal of Biological Chemistry. vol. 268, No. 26, pp. 19228-19231. cited by other .
Fowler, Bruce O., Milenko Markovi , and Walter E. Brown. 1993. "Octacalcium Phosphate. 3. Infrared and Raman Vibrational Spectra." Chem. Mater. vol. 5, No. 10, pp. 1417-1423. cited by other .
Fuhrhop, Jurgen-Hinrich, Dragan Spiroski, and Christoph Boettcher. 1993. "Molecular Monolayer Rods and Tubules Made of .alpha.-(L-Lysine), .omega.-(Amino) Bolaamphiphiles." Journal of the American Chemical Society. vol. 115, No. 4, pp. 1600-1601. cited by other .
Graham, Stephan and Paul W. Brown. 1993. "The Low Temperature Formation of Octacalcium Phosphate." Journal of Crystal Growth. vol. 132, pp. 215-225. cited by other .
Stupp, Samuel I., Jacqueline A. Hanson, Jo Ann Eurell, Glenn W. Ciegler, and Ann Johnson. 1993. "Organoapatites: Materials for Artificial Bone. III. Biological Testing." Journal of Biomedical Materials Reasearch. vol. 27, pp. 301-311. cited by other .
Stupp, Samuel I., George C. Mejicano, and Jacqueline A. Hanson. 1993. "Organoapatites: Materials for Artificial Bone. II. Hardening Reactions and Properties." Journal of Biomedical Materials Research. vol. 27, pp. 289-299. cited by other .
Wald, Heidi L., Georgios Sarakinos, Michelle D. Lyman, Antonios G. Mikos, Joseph P. Vacanti, and Robert Langer. 1993. "Cell Seeding in Porous Transplantation Devices." Biomaterials. vol. 14, No. 4, pp. 270-278. cited by other .
Walsh, Dominic, Joanne L. Kingston, Brigid R. Heywood, and Stephen Mann. 1993. "Influence of Monosaccharides and Related Molecules on the Morphology of Hydroxyapatite." Journal of Crystal Growth. vol. 133, pp. 1-12. cited by other .
Wang, B. C., T. M. Lee, E. Chang, and C. Y. Yang. 1993. "The Shear Strength and the Failure Mode of Plasma-Sprayed Hydroxyapatite Coating to Bone: The Effect of Coating Thickness." Journal of Biomedical Materials Research. vol. 27, pp. 1315-1327. cited by other .
San Antonio, James D., Arthur D. Lander, Morris J. Karnovsky, and Henry S. Slayter. Jun. 1994. "Mapping the Heparin-Binding Sites on Type I Collagen Monomers and Fibrils." The Journal of Cell Biology. vol. 125, No. 5, pp. 1179-1188. cited by other .
Ban, S., S. Maruno, H. Iwata, and H. Itoh. 1994. "Calcium Phosphate Precipitation on the Surface of HA-G-Ti Composite Under Physiologic Conditions." Journal of Biomedical Materials Research. vol. 28, pp. 65-71. cited by other .
de Bruijn, J. D., Y. P. Bovell, and C. A. van Blitterswijk. 1994. "Structural Arrangements at the Interface Between Plasma Sprayed Calcium Phosphates and Bone." Biomaterials. vol. 15, No. 7, pp. 543-550. cited by other .
Hunter, Graeme K. and Harvey A. Goldberg. 1994. "Modulation of Crystal Formation by Bone Phosphoproteins: Role of Glutamic Acid-Rich Sequences in the Nucleation of Hydroxyapatite by Bone Sialoprotein." Biochem. J. vol. 302, pp. 175-179. cited by other .
Klein, C. P. A. T., J. G. C. Wolke, J. M. A. de Blieck-Hogervorst, and K. de Groot. 1994. "Calcium Phosphate Plasma-Sprayed Coatings and Their Stability: An in Vivo Study." Journal of Biomedical Materials Research. vol. 28, pp. 909-917. cited by other .
Mikos, Antonios G., Michelle D. Lyman, Lisa E. Freed, and Robert Langer. 1994. "Wetting of Poly(L-Lactic Acid) and Poly(DL-Lactic-co-glycolic Acid) Foams for Tissue Culture." Biomaterials. vol. 15, No. 1, pp. 55-58. cited by other .
Bond, G. M., R. H. Richman, and W. P. McNaughton. Jun. 1995. "Mimicry of Natural Material Designs and Processes." Journal of Materials Engineering and Performance. vol. 4, No. 3, pp. 334-345. cited by other .
Hubbell, Jeffrey A. Jun. 1995. "Biomaterials in Tissue Engineering." Bio/technology. vol. 13, pp. 565-576. cited by other .
Fromm, J. R., R. E. Hileman, E. E. O. Caldwell, J. M. Weiler, and R. J. Linhardt. Nov. 10, 1995. "Differences in the Interaction of Heparin to Acidic Fibroblast Growth Factor." Archives of Biochemistry and Biophysics. vol. 323, No. 2, pp. 279-287. cited by other .
Wakitani, Shigeyuki, Tomoyuki Saito, and Arnold I. Caplan. Dec. 1995. "Myogenic Cells Derived from Rat Bone Marrow Mesenchymal Stem Cells Exposed to 5-Azacytidine." Muscle & Nerve. vol. 18, pp. 1417-1426. cited by other .
Aletras, Alexios, Kleomenis Barlos, Dimitrios Gatos, Sophia Koutsogianni, and Petros Mamos. 1995. "Preparation of the Very Acid-Sensitive Fmoc-Lys(Mtt)-OH." International Journal of Peptide & Protein Research. vol. 45, pp. 488-496. cited by other .
Berndt, Peter, Gregg B. Fields, and Matthew Tirrell. 1995. "Synthetic Lipidation of Peptides and Amino Acids: Monolayer Structure and Properties." Journal of the American Chemical Society. vol. 117, No. 37, pp. 9515-9522. cited by other .
Gage, Fred H., Jasodhara Ray, and Lisa J. Fisher. 1995. "Isolation, Characterization, and Use of Stem Cells from the CNS." Annual Review of Neuroscience. vol. 18, pp. 159-192. cited by other .
Nomizu, Motoyoshi, Benjamin S. Weeks, Christi A. Weston, Woo Hoo Kim, Hynda K. Kleinman, and Yoshihiko Yamada. 1995. "Structure-Activity Study of a Laminin .alpha.1 Chain Active Peptide Segment Ile-Lys-Val-Ala-Val (IKVAV)." FEBS Letters. vol. 365, pp. 227-231. cited by other .
Saito, Tomoyuki, James E. Dennis, Donald P. Lennon, Randell G. Young, and Arnold I. Caplan. 1995. "Myogenic Expression of Mesenchymal Stem Cells Within Myotubes of mdx Mice in Vitro and in Vivo." Tissue Engineering. vol. 1, No. 4, pp. 327-343. cited by other .
Sasanuma, Michio. 1995. "Optical Processes in ZnO." J. Phys.: Condens. Matter. vol. 7, pp. 10029-10036. cited by other .
Zhang, Shuguang, Todd C. Holmes, C. Michael DiPersio, Richard O. Hynes, Xing Su, and Alexander Rich. 1995. "Self-Complementary Oligopeptide Matrices Support Mammalian Cell Attachment." Biomaterials. vol. 16, No. 18, pp. 1385-1393. cited by other .
Falini, Guiseppe, Shira Albeck, Steve Weiner, and Lia Addadi. Jan. 5, 1995. "Control of Aragonite or Calcite Polymorphism by Mollusk Shell Macromolecules." Science. vol. 271, No. 5245, pp. 67-69. cited by other .
Alivisatos, A. P. Feb. 16, 1996. "Semiconductor Clusters, Nanocrystals, and Quantum Dots." Science. vol. 271, No. 5251, pp. 933-937. cited by other .
Keyt, Bruce A., Lea T. Berleau, Hung V. Nguyen, Helen Chen, Henry Heinsohn, Richard Vandlen, and Napoleone Ferrara. Mar. 29, 1996. "The Carboxyl-terminal Domain (111-165) of Vascular Endothelial Growth Factor Is Critical for Its Mitogenic Potency." The Journal of Biological Chemistry. vol. 271, No. 13, pp. 7788-7795. cited by other .
Belcher, A. M., X. H. Wu, R. J. Christensen, P. K. Hansma, G. D. Stucky, and D. E. Morse. May 2, 1996. "Control of Crystal Phase Switching and Orientation by Soluble Mullusc-Shell Proteins." Nature. vol. 381, pp. 56-58. cited by other .
Hortelano, Gonzalo, Ayman Al-Hendy, Frederick A. Ofosu, and Patricia L. Chang. Jun. 15, 1996. "Delivery of Human Factor IX in Mice by Encapsulated Recombinant Myoblasts: A Novel Approach Towards Allogenic Gene Therapy of Hemophilia B." Blood. vol. 87, No. 12, pp. 5095-5103. cited by other .
Sultzbaugh, K. J. and T. J. Speaker. Jul.-Aug. 1996. "A Method to Attach Lectins to the Surface of Spermine Alginate Microcapsules Based on the Avidin Biotin Interaction." J. Microencapsulation. vol. 13, No. 4, pp. 363-375. cited by other .
Alivisatos, A. Paul, Kai P. Johnsson, Xiaogang Peng, Troy E. Wilson, Colin J. Loweth, Marcel P. Burchez Jr., and Peter G. Schultz. Aug. 15, 1996. "Organization of `Nanocrystal Molecules` Using DNA." Nature. vol. 382, pp. 609-611. cited by other .
George, Anne, Leslie Bannon, Boris Sabsay, Jerry W. Dillon, James Malone, Arthur Veis, Nancy A. Jenkins, Debra J. Gilbert, and Neal G. Copeland. Dec. 20, 1996. "The Carboxyl-terminal Domain of Phosphophoryn Contains Unique Extended Triplet Amino Acid Repeat Sequences Forming Ordered Carboxyl-Phosphate Interaction Ridges That May Be Essential in the Biomineralization Process." The Journal of Biological Chemistry. vol. 271, No. 51, pp. 32869-32873. cited by other .
Basso, D. Michele, Michael S. Beattie, and Jacqueline C. Bresnahan. 1996. "Graded Histological and Locomotor Outcomes after Spinal Cord Contusion Using the NYU Weight-Drop Device Versus Transectiom." Experimental Neurology. vol. 139, pp. 244-256. cited by other .
Burkett, Sandra L. and Stephen Mann. 1996. "Spatial Organization and Patterning of Gold Nanoparticleson Self-Assembled Biolipid Tubular Templates." Chem. Commun. pp. 321-322. cited by other .
Hunter, Graeme K., Peter V. Hauschka, A. Robin Poole, Lawrence C. Rosenberg, and Harvey A. Goldberg. 1996. "Nucleation and Inhibition of Hydroxyapatite Formation by Mineralized Tissue Proteins." Biochem. J. vol. 317, pp. 59-64. cited by other .
Karymov, Mikhail A., Karel Prochazka, John M. Mendenhall, Thomas J. Martin, Petr Munk, and Stephen E. Webber. 1996. "Chemical Attachment of Polystyrene-block-poly(methacrylic acid) Micelles on a Silicon Nitride Surface." Langmuir. vol. 12, No. 20, 4748-4753. cited by other .
Landis, William J., Karen J. Hodgens, James Arena, Min Ja Song, and Bruce F. McEwen. 1996. "Structural Relations Between Collagens and Mineral in Bone as Determined by High Voltage Electron Microscopic Tomography." Microscopy Research and Technique. vol. 33, pp. 192-202. cited by other .
Matsuzawa, Mieko, Forrest F. Weight, Richard S. Potember, and Paivi Liesi. 1996. "Directional Neurite Outgrowth and Axonal Differentiation of Embryonic Hippocampal Neurons Are Promoted by a Neurite Outgrowth Domain of the B2-Chain of Laminin." Int. J. Devl. Neuroscience. vol. 14, No. 3, pp. 283-295. cited by other .
Mooney, David J., Daniel F. Baldwin, Nam P. Suh, Joseph P. Vacanti, and Robert Langer. 1996. "Novel Approach to Fabricate Porous Sponges of Poly(D,L-Lactic-co-glycolic Acid) Without the Use of Organic Solvents." Biomaterials. vol. 17, No. 14, pp. 1417-1422. cited by other .
Ratner, Buddy D., Allan S. Hoffman, Frederick J. Schoen, and Jack E. Lemons, Editors. 1996. Biomaterials Science: An Introduction to Materials in Medicine. San Diego, CA: Academic Press. cited by other .
Ulman, Abraham. 1996. "Formation and Structure of Self-Assembled Monolayers." Chemical Reviews. vol. 96, No. 4, pp. 1533-1554. cited by other .
Zarif, Leila, Ange Polidori, Bernard Pucci, Tadek Gulik-Krzywicki, Andre A, Pavia, and Jean G. Reiss. 1996. "Effect of Chirality on the Formation of Tubules from Glycolipidic Amphiphiles." Chemistry and Physics of Lipids. vol. 79, pp. 165-170. cited by other .
Aggeli, A., M. Bell, N. Boden, J. N. Keen, P. F. Knowles, T. C. B. McLeish, M. Pitkeathly, and S. E. Radford. Mar. 20, 1997. "Responsive Gels Formed by the Spontaneous Self-Assembly of Peptides into Polymeric .beta.-Sheet Tapes." Nature. vol. 386, pp. 259-262. cited by other .
Herr Andrew B., David M. Ornitz, Ram Sasisekharan, Ganesh Venkataraman, and Gabriel Waksman. Jun. 27, 1997. "Heparin-Induced Self-Association of Fibroblast Growth Factor-2." The Journal of Biological Chemistry. vol. 272, No. 26, pp. 16382-16389. cited by other .
Dimmeler, Stefanie and Andreas M. Zeiher. Aug. 1997. "Nitric Oxide and Apoptosis: Another Paradigm for the Double-Edged Role of Nitric Oxide." Nitric Oxide: Biology and Chemistry. vol. 1, No. 4, pp. 275-281. cited by other .
Stupp, Samuel I. and Paul V. Braun. Aug. 29, 1997. "Molecular Manipulation of Microstructures: Biomaterials, Ceramics, and Semiconductors." Science. vol. 277, No. 5330, pp. 1242-1248. cited by other .
Kaufmann, P. M., S. Heimrath, B. S. Kim, and D. J. Mooney. Sep./Oct. 1997. "Highly Porous Polymer Matrices as a Three-Dimensional Culture System for Hepatocytes." Cell Transplantation. vol. 6, No. 5, pp. 463-468. cited by other .
Aggeli, Amalia, Mark Bell, Neville Boden, Jeff N. Keen, Tom C. B. McLeish, Irina Nyrkova, Sheena E. Radford, and Alexander Semenov. 1997. "Engineering of Peptide .beta.-Sheet Nanotapes." J. Mater. Chem. vol. 7, No. 7, pp. 1135-1145. cited by other .
Anderson, James M. and Matthew S. Shive. 1997. "Biodegradation and Biocompatibility of PLA and PLGA Microspheres." Advanced Drug Delivery Reviews. vol. 28, pp. 5-24. cited by other .
Draget, Kurt Ingar, Gudmund Skjak-Br.ae butted.k, Olav Smidsod. 1997. "Alginate Based New Materials." International Journal of Biological Macromolecules. vol. 21, pp. 47-55. cited by other .
El-Ghannam, Ahmed, Paul Ducheyne, and Irving M. Shapiro. 1997. "Porous Bioactive Glass and Hydroxyapatite Ceramic Affect Bone Cell Function In Vitro Along Different Time Lines." Journal of Biomedical Materials Research. vol. 36, pp. 167-180. cited by other .
Jaiswal, Neelam, Stephen E. Haynesworth, Arnold I. Caplan, and Scott P. Bruder. 1997. "Osteogenic Differentiation of Purified, Culture-Expanded Human Mesenchymal Stem Cells In Vitro." Journal of Cellular Biochemistry. vol. 64, pp. 295-312. cited by other .
Nehrer, Stefan, Howard A. Breinan, Arun Ramappa, Sonya Shortkoff, Gretchen Young, Tom Minas, Clement B. Sledge, Ioannis V. Yannas, and Myron Spector. 1997. "Canine Chondrocytes Seeded in Type I and Type II Collagen Implants Investigated In Vitro." Journal of Biomedical Materials Research (Appl. Biomater.). vol. 38, pp. 95-104. cited by other .
Mann, Stephen. 1997. "Biomineralization: The Form(id)able Part of Bioinorganic Chemistry!" J. Chem. Soc., Dalton Trans. pp. 3953-3961. cited by other .
Norrby, Klas. 1997. "Angiogenesis: New Aspects Relating to Its Initiation and Control." APMIS. vol. 105, pp. 417-437. cited by other .
Shimizu, Toshimi, Masaki Kogiso, and Mitsutoshi Masuda. 1997. "Noncovalent Formation of Polyglycine II-Type Structure by Hexagonal Self-Assembly of Linear Polymolecular Chains." Journal of the American Chemical Society. vol. 119, No. 26, pp. 6209-6210, S2-S17. cited by other .
Smith, George P. and Valery A. Petrenko. 1997. "Phage Display." Chemical Reviews. vol. 97, No. 2, pp. 391-410. cited by other .
Toyotama, Akiko, Shin-ichi Kugimiya, Masakatsu Yonese, Takatoshi Kinoshita, and Yoshiharu Tsujita. 1997. "Controllable Orientation of the Peptide-Based Surfactant at Air-Water Interface." Chemistry Letters. pp. 443-444. cited by other .
Weiner, Stephen and Lia Addadi. 1997. "Design Strategies in Mineralized Biological Materials." J. Mater. Chem. vol. 7, No. 5, pp. 689-702. cited by other .
Wellings, Donald A. and Eric Atherton. 1997. "Standard Fmoc Protocols." Methods in Enzymology. vol. 289, pp. 44-67. cited by other .
Wen, H. B., J. G. C. Wolke, J. R. de Wijn, Q. Liu, F. Z. Cui, and K. de Groot. 1997. "Fast Precipitation of Calcium Phosphate Layers on Titanium Induced by Simple Chemical Treatments." Biomaterials. vol. 18, No. 22, pp. 1471-1478. cited by other .
Yu, Ying-Ching, Teika Pakalns, Yoav Dori, James B. McCarthy, Matthew Tirrell, and Gregg B. Fields. 1997. "Construction of Biologically Active Protein Molecular Architecture Using Self-Assembling Peptide-Amphiphiles." Methods in Enzymology. vol. 289, pp. 571-587. cited by other .
Zhitomirsky, I. and l. Gal-Or. 1997. "Electrophoretic Deposition of Hydroxyapatite." Journal of Materials Science: Materials in Medicine. pp. 213-219. cited by other .
Veis, Arthur, Kuiru Wei, Charles Sfeir, Anne George, and James Malone. Jan. 1998. "Properties of the (DSS).sub.n Triplet Repeat Domain of Rat Dentin Phosphoryn." European Journal of Oral Sciences. vol. 106 (suppl. 1), pp. 234-238. cited by other .
Pincus, David W., Robert R. Goodman, Richard A. R. Fraser, Maiken Nedergaard, and Steven A. Goldman. Apr. 1998. "Neural Stem and Progenitor Cells: A Strategy for Gene Therapy and Brain Repair." Neurosurgery. vol. 42, No. 4, pp. 858-867. cited by other .
Ogiso, M., and Y. Yamashita, and T. Matsumoto. Jun. 1998. "The Process of Physical Weakening and Dissolution of the HA-Coated Implant in Bone and Soft Tissue." Journal of Dental Research. vol. 77, No. 6, pp. 1426-1434. cited by other .
Petka, Wendy A., James L. Harden, Kevin P. McGrath, Denis Wirtz, and David A. Tirrell. Jul. 17, 1998. "Reversible Hydrogels from Self-Assembling Artificial Proteins." Science. vol. 281, No. 5375, pp. 389-392. cited by other .
Orgill, Dennis P., Charles Bulter, John F. Regan, Mark S. Barlow, I. V. Yannas, and Carolyn C. Compton. Aug. 1998. "Vascularized Collagen-Glycosaminoglycan Matrix Provides a Dermal Substrate and Improves Take of Cultured Epithelial Autografts." Plastic and Reconstructive Surgery. vol. 102, No. 2, pp. 423-429. cited by other .
Yu, Ying-Ching, Matthew Tirrell, and Gregg B. Fields. Oct. 7, 1998. "Minimal Lipidation Stabilizes Protein-Like Molecular Architecture." Journal of the American Chemical Society. vol. 120, No. 39, pp. 9979-9987. cited by other .
Borkenhagen, M., J.-F. Clemence, H. Sigrist, and P. Aebischer. 1998. "Three-Dimensional Extracellular Matrix Engineering in the Nervous System." Journal of Biomedical Materials Research. vol. 40, pp. 392-400. cited by other .
Brekke, John H. and Jeffrey M. Toth. 1998. "Principles of Tissue Engineering Applied to Programmable Osteogenesis." Journal of Biomedical Materials Research (Appl. Biomater.). vol. 43, pp. 380-398. cited by other .
Fields, Gregg B., Janelle L. Lauer, Yoav Dori, Pilar Forns, Ying-Ching Yu, and Matthew Tirrell. 1998. "Proteinlike Molecular Architecture: Biomaterial Applications for Inducing Cellular Receptor Binding and Signal Transduction." Biopolymers (Peptide Science), vol. 47, pp. 143-151. cited by other .
Gu, Keni, Syweren R. Chang, Matt S. Slaven, Brian H. Clarkson, R. Bruce Rutherford, and Helena H. Ritchie. 1998. "Human Dentin Phosphophoryn Nucleotide and Amino Acid Sequence." European Journal of Oral Sciences. vol. 106, pp. 1043-1047. cited by other .
Hartgerink, Jeffrey D., Thomas D. Clark, and M. Reza Ghadiri. 1998. "Peptide Nanotubes and Beyond." Chem. Eur. J. vol. 4, No. 8, pp. 1367-1372. cited by other .
Johnstone, Brian, Thomas M. Hering, Arnold I. Caplan, Victor M. Goldberg, and Jung U. Yoo. 1998. "In Vitro Chondrogenesis of Bone Marrow-Derived Mesenchymal Progenitor Cells." Experimental Cell Research. vol. 238, pp. 265-272. cited by other .
Kawasaki, M., A. Ohtomo, I. Ohkubo, H. Koinuma, Z. K. Tang, P. Yu, G. K. L. Wong, B. P. Zhang, and Y. Segawa. 1998. "Excitonic Ultraviolet Laser Emission at Room Temperature from Naturally Made Cavity in ZnO Nanocrytal Thin Films." Materials Science and Engineering. vol. B56, pp. 239-245. cited by other .
Kogiso, Masaki, Satomi Ohnishi, Kiyoshi Yase, Mitsutoshi Masuda, and Toshimi Shimizu. 1998. "Dicarboxylic Oligopeptide Bolaamphiphiles: Proton-Triggered Self-Assembly of Microtubes with Loose Solid Surfaces." Langmuir. vol. 14, No. 18, pp. 4978-4986, S1-S7. cited by other .
Kogiso, Masaki, Takeshi Hanada, Kiyoshi Yase, and Toshimi Shimizu, 1998. "Intralayer Hydrogen-Bond-Directed Self-Assembly of Nano-Fibers from Dicarboxylic Valylvaline Bolaamphiphiles." Chem. Comm. pp. 1791-1792. cited by other .
Li, Panjian and Paul Ducheyne. 1998. "Quasi-Biological Apatite Film Induced by Titanium in a Simulated Body Fluid." Journal of Biomedical Materials Research. vol. 41, pp. 341-348. cited by other .
Nanci, A., J. D. Wuest, L. Peru, P. Brunet, V. Sharma, S. Zalzal, and M. D. McKee. 1998. "Chemical Modification of Titanium Surfaces for Covalent Attachment of Biological Molecules." Journal of Biomedical Materials Research. vol. 40, pp. 324-335. cited by other .
Tsui, Y. C., C. Doyle, and T. W. Clyne. 1998. "Plasma Sprayed Hydroxyapatite Coatings on Titanium Substrates Part 2: Optimisation of Coating Properties." Biomaterials. vol. 19, pp. 2031-2043. cited by other .
Weiner, S. and H. D. Wagner. 1998. "The Material Bone: Structure-Mechanical Function Relations." Annu. Rev. Mater. Sci. vol. 28, pp. 271-298. cited by other .
Wen, H. B., J. R. de Wijn, F. Z. Cui, and K. de Groot. 1998. "Preparation of Calcium Phosphate Coatings on Titanium Implant Materials by Simple Chemistry." Journal of Biomedical Materials Research. vol. 41, pp. 227-236. cited by other .
Wheeler, Donna L., David L. Chamberland, John M. Schmitt, David C. Buck, John H. Brekke, Jeffrey O. Hollinger, S.-P. Joh, and K.-W. Suh. 1998. "Radiomorphometry and Biomechanical Assessment of Recombinant Human Bone Morphogenetic Protein 2 and Polymer in Rabbit Radius Ostectomy Model." Journal of Biomedical Materials Research (Appl. Biomater.). vol. 43, pp. 365-373. cited by other .
Wolke, J. G. C., K. de Groot, and J. A. Jansen. 1998. "In Vivo Dissolution Behavior of Various RF Magnetron Sputtered Ca-P Coatings." Journal of Biomedical Materials Research. vol. 39, pp. 524-530. cited by other .
Xiao, Shou-Jun, Marcus Textor, and Nicholas D. Spencer. 1998. "Covalent Attachment of Cell-Adhesive, (Arg-Gly-Asp)-Containing Peptides to Titanium Surfaces." Langmuir. vol. 14, No. 19, pp. 5507-5516. cited by other .
Xu, Guofeng, Nan Yao, Ilhan A. Aksay, and John T. Groves. 1998. "Biomimetic Synthesis of Macroscopic-Scale Calcium Carbonate Thin Films. Evidence for a Multistep Assembly Process." Journal of the American Chemical Society. vol. 120, No. 46, pp. 11977-11985. cited by other .
Yamada, Norihiro, Katsuhiko Ariga, Masanobu Naito, Kazuhiro Matsubara, and Emiko Koyama. 1998. "Regulation of .beta.-Sheet Structures Within Amyloid-Like .beta.-Sheet Assemblage from Tripeptide Derivatives." Journal of the American Chemical Society. vol. 120, No. 47, pp. 12192-12199. cited by other .
Chusuei, Charles C., D. Wayne Goodman, Michael J. Van Stipdonk, Dina R. Justes, and Emile A. Schweikert. Jan. 1, 1999. "Calcium Phosphate Phase Identification Using XPS and Time-of-Flight Cluster SIMS." Analytical Chemistry. vol. 71, No. 1, pp. 149-153. cited by other .
Zubarev, Eugene R., Martin U. Pralle, Leiming Li, and Samuel I. Stupp. Jan. 22, 1999. "Conversion of Supramolecular Clusters to Macromolecular Objects." Science. vol. 283, pp. 523-526. cited by other .
Won, You-Yeon, H. Ted Davis, and Frank S. Bates. Feb. 12, 1999. "Giant Wormlike Rubber Micelles." Science. vol. 283, No. 5404, pp. 960-963. cited by other .
Corral, Claudio J., Aamir Siddiqui, Liancun Wu, Catherine l. Farrell, David Lyons, and Thomas A. Mustoe. Feb. 1999. "Vascular Endothelial Growth Factor Is More Important Than Basic Fibroblastic Growth Factor During Ischemic Wound Healing." Arch. Surg. vol. 134, pp. 200-205. cited by other .
Wheeler, B. C., J. M. Corey, G. J. Brewer, and D. W. Branch. Feb. 1999. "Microcontact Printing for Precise Control of Nerve Cell Growth in Culture." Journal of Biomechanical Engineering. vol. 121, pp. 73-78. cited by other .
Cao, H., Y. G. Zhao, S. T. Ho, E. W. Seelig, Q. H. Wang, and R. P. H. Chang. Mar. 15, 1999. "Random Laser Action in Semiconductor Powder." Physical Review Letters. vol. 82, No. 11, pp. 2278-2281. cited by other .
Aizenberg, Joanna, Andrew J. Black, and George M. Whitesides. Apr. 8, 1999. "Control of Crystal Nucleation by Patterned Self-Assembled Monolayers." Nature. vol. 398, pp. 495-498. cited by other .
Niklason, L. E., J. Gao, W. M. Abbott, K. K. Hirschi, S. Houser, R. Marini, and R. Langer. Apr. 16, 1999. "Functional Arteries Grown in Vitro." Science. vol. 284, pp. 489-493. cited by other .
Hahn, Jungseok and Stephen E. Webber. Apr. 1999. "Modification of Surfaces by Covalent Attachment of Polymer Micelles." Macromolecular Symposia. vol. 139, pp. 39-47. cited by other .
Liu, Yi, Duckhyun Kim, B. Timothy Himes, Stella Y. Chow, Timothy Schallert, Marion Murray, Alan Tessler, and Itzhak Fischer. Jun. 1, 1999. "Transplants of Fibroblasts Genetically Modified to Express BDNF Promote Regeneration of Adult Rat Rubrospinal Axons and Recovery of Forelimb Function." The Journal of Neuroscience. vol. 19, No. 11, pp. 4370-4387. cited by other .
Mehler, Mark F. and John A. Kessler. Jul. 1999. "Progenitor Cell Biology: Implications for Neural Regeneration." Arch. Neurol. vol. 56, pp. 780-784. cited by other .
Tirrell, M. Oct. 27, 1999. "Biofunctionalization of Surfaces with Peptide Amphiphiles." AVS: Science & Technology. Invited Paper BI-WeM7. cited by other .
McDonald, John W., Xiao-Zhong Liu, Yun Qu, Su Liu, Shannon K. Mickey, Dorothy Turetsky, David I. Gottlieb, and Dennis W. Choi. Dec. 1999. "Transplanted Embryonic Stem Cells Survive, Differentiate and Promote Ecovery in Injured Rat Spinal Cord." Nature Medicine. vol. 5, No. 12, pp. 1410-1412. cited by other .
Bradt, Jens-Hilmar, Michael Mertig, Angelika Teresiak, and Wolfgang Pompe. 1999. "Biomimetic Mineralization of Collagen by Combined Fibril Assembly and Calcium Phosphate Formation." Chem. Mater. vol. 11, No. 10, pp. 2694-2701. cited by other .
Braun, Paul V. and Samuel I. Stupp. 1999. "CdS Mineralization of Hexagonal, Lamellar, and Cubic Lyotropic Liquid Crystals." Materials Research Bulletin. vol. 34, No. 3, pp. 463-469. cited by other .
Bulter, C. E., I. V. Yannas, C. C. Compton, C. A. Correia, and D. P. Orgill. 1999. "Comparison of Cultured and Uncultured Keratinocytes Seeded into a Collagen-GAG Matrix for Skin Replacements." British Journal of Plastic Surgery. vol. 52, pp. 127-132. cited by other .
Chai, C. S. and B. Ben-Nissan. 1999. "Bioactive Nanocrystalline Sol-Gel Hydroxyapatite Coatings." Journal of Materials Science: Materials in Medicine. vol. 10, pp. 465-469. cited by other .
Clark, Thomas D., Kenji Kobayashi, and M. Reza Ghadiri. 1999. "Covalent Capture and Stabilization of Cylindrical .beta.-Sheet Peptide Assemblies." Chem. Eur. J. vol. 5, No. 2, pp. 782-792. cited by other .
Cornish, J., K. E. Callon, C. Q.-X. Lin, C. L. Xiao, T. B. Mulvey, G. J. S. Cooper, and I. R. Reid. 1999. "Trifluoroacetate, a Contaminant in Purified Proteins, Inhibits Proliferation of Osteoblasts and Chondrocytes." Am. J. Physiol. Endocrinol. Metab. vol. 277, pp. 779-783. cited by other .
Emoto, Kazunori, Yukio Nagasaki, and Kazunori Kataoka. 1999. "Coating of Surfaces with Stabilized Reactive Micelles from Poly(ethylene glycol)--Poly(DL-Lactic Acid) Block Copolymer." Langmuir. vol. 15, No. 16, pp. 5212-5218. cited by other .
Fields, Gregg B. 1999. "Induction of Protein-like Molecular Architecture by Self-Assembly Processes." Bioorganic & Medicinal Chemistry. vol. 7, pp. 75-81. cited by other .
Haynes, Andrew J., Wei-Qun Huang, Jamie Mallah, Dajun Yang, Marc E. Lippman, and Lu-Yuan Li. 1999. "Angiopoietin-I and Its Receptor Tie-2 Participate in the Regulation of Capillar-like Tubule Formation and Survival of Endothelial Cells." Microvascular Research. vol. 58, pp. 224-237. cited by other .
Hwang, Julia J., Kevin Jaeger, James Hancock, and Samuel I. Stupp. 1999. "Organoapatite Growth on an Orthopedic Alloy Surface." Journal of Biomedical Materials Research. vol. 47, pp. 504-515. cited by other .
Ignjatovi , Nenad, Simonida Torni , Mom{hacek over (c)}ilo Daki , Miroslav Miljkovi , Milenko Plav{hacek over (s)}i , and Dragan Uskskovi . 1999. "Synthesis and Properties of Hyrdroxyapatite/Poly-L-Lactide Composite Biomaterials." Biomaterials. vol. 20, pp. 809-816. cited by other .
Lee, Kevin J. and Thomas M. Jessell. 1999. "The Specification of Dorsal Cell Fates in the Vertebrate Central Nervous System." Annual Review of Neuroscience. vol. 22, pp. 261-294. cited by other .
Lee, Kyujin C., Paul A. Carlson, Alex S. Goldstein, Paul Yager, and Michael H. Gelb. 1999. "Protection of a Decapeptide from Proteolytic Cleavage by Lipidation and Self-Assembly into High-Axial-Ratio Microstructures: A Kinetic and Structural Study." Langmuir. vol. 15, No. 17, pp. 5500-5508. cited by other .
Mao, Chuanbin, Hengde Li, Fuzhai Cui, Chunlai Ma, and Qinglin Feng. 1999. "Oriented Growth of Phosphates on Polycrystalline Titanium in a Process Mimicking Biomineralization." Journal of Crystal Growth. vol. 206, pp. 308-321. cited by other .
Miyaji, Fumiaki, Hyun-Min Kim, Shinichi Handa, Tadashi Kokubo, and Takashi Nakamura. 1999. "Bonelike Apatite Coating on Organic Polymers: Novel Nucleation Process Using Sodium Silicate Solution." Biomaterials. vol. 20, pp. 913-919. cited by other .
Pakalns, Teika, Kraig L. Haverstick, Gregg B. Fields, James B. McCarthy, Daniel L. Mooradian, and Matthew Titrrell. 1999. "Cellular Recognition of Synthetic Peptide Amphiphiles in Self- Assembled Monolayer Films." Biomaterials. vol. 20, pp. 2265-2279. cited by other .
Pittenger, Mark F., Alastair M. Mackay, Stephen C. Beck, Rama K. Jaiswal, Robin Douglas, Joseph D. Mosca, Mark A. Moorman, Donald W. Simonetti, Stewart Craig, and Daniel R. Marshak. Apr. 2, 1999. "Multilineage Potential of Adult Human Mesenchymal Stem Cells." Science. vol. 284, pp. 143-147. cited by other .
Rezania, Alireza, Robert Johnson, Anthony R. Lefkow, and Kevin E. Healy. 1999. "Bioactivation of Metal Oxide Surfaces. 1. Surface Characterization and Cell Response." Langmuir. vol. 15, No. 20, pp. 6931-6939. cited by other .
Rowley, Jon A., Gerard Madlambayan, and David J. Mooney. 1999. "Alginate Hydrogels as Synthetic Extracellular Matrix Materials." Biomaterials. vol. 20, pp. 45-53. cited by other .
Schense, Jason C. and Jeffrey A. Hubbell. 1999. "Cross-Linking Exogenous Bifunctional Peptides into Fibrin Gels with Factor XIIIa." Bioconjugate Chem. vol. 10, No. 1, pp. 75-81. cited by other .
Varma, H. K., Y. Yokogawa, F. F. Espinosa, Y. Kawamoto, K. Nishizawa, F. Nagata, and T. Kameyama. 1999. "In-Vitro Calcium Phosphate Growth over Functionalized Cotton Fibers." Journal of Materials Science: Materials in Medicine. vol. 10, pp. 395-400. cited by other .
Vernon, Robert B. and E. Helene Sage. 1999. "A Novel, Quantitative Model for Study of Endothelial Cell Migration and Sprout Formation Within Three-Dimensional Collagen Matrices." Microvascular Research. vol. 57, pp. 188-133. cited by other .
Wei, M., A. J. Ruys, M. V. Swain, S. H. Kim, B. K. Milthorpe, and C. C. Sorrell. 1999. "Interfacial Bond Strength of Electrophoretically Deposited Hydroxyapatite Coatings on Metals." Journal of Materials Science: Materials in Medicine. vol. 10, pp. 401-409. cited by other .
Yu, Ying-Ching, Vikram Roontga, Vladimir A. Daragan, Kevin H. Mayo, Matthew Tirrell, and Gregg B. Fields. 1999. "Structure and Dynamics of Peptide--Amphiphiles Incorporating Triple-Helical Proteinlike Molecular Architecture." Biochemistry. vol. 38, No. 5, pp. 1659-1668. cited by other .
Huq, N. Laila, Keith J. Cross, and Eric C. Reynolds. Feb. 4, 2000. "Molecular Modelling of a Multiphosphorylated Sequence Motif Bound to Hydroxyapatite Surfaces." Journal of Molecular Modeling. vol. 6, pp. 35-47. cited by other .
Martinez, J. S., G. P. Zhang, P. D. Holt, H.-T,Jung, C. J. Carrano, M. G. Haygood, and Alison Butler. Feb. 18, 2000. "Self-Assembling Amphiphilic Siderophores from Marine Bacteria." Science. vol. 287, No. 5456, pp. 1245-1247. cited by other .
Verrecchio, Angela, Markus W. Germann. Barbara P. Schick, Brian Kung, Thomas Twardowski, and James D. San Antonio. Mar. 17, 2000. "Design of Peptides with High Affinites for Heparin and Endothelial Cell Proteoglycans." The Journal of Biological Chemistry. vol. 275, No. 11, pp. 7701-7707. cited by other .
Cao, H., J. Y. Xu, E. W. Seelig, and R. P. H. Chang. May 22, 2000. "Microlaser Made of Disordered Media." Applied Physics Letters. vol. 76, No. 21, pp. 2997-2999. cited by other .
Marler, Jennifer J., Amrita Guha, Jonathan Rowley, Rahul Koka, David Mooney, Joseph Upton, and Joseph Vacanti. May 2000. "Soft-Tissue Augmentation with Injectable Alginate and Syngeneic Fibroblasts." Plastic and Reconstructive Surgery. vol. 105, No. 6, pp. 2049-2058. cited by other .
Holmes, Todd C., Sonsoles de Lacalle, Xing Su, Guosong Liu, Alexander Rich, and Shuguang Zhang. Jun. 6, 2000. "Extensive Neurite Outgrowth and Active Synapse Formation on Self-Assembling Peptide Scaffolds." Proceedings of the National Academy of Sciences of the United States of America. vol. 97, No. 12, pp. 6728-6733. cited by other .
Whaley, Sandra R., D. S. English, Evelyn L. Hu, Paul F. Barbara, and Angela M. Belcher. Jun. 8, 2000. "Selection of Peptides with Semiconductor Binding Specificity for Directed Nanocrystal Assembly." Nature. vol. 405, pp. 665-668. cited by other .
Sun, Xiu-xia and Chi-chen Wang. Jul. 28, 2000. "The N-Terminal Sequence (Residues 1-65) Is Essential for Dimerization, Activities, and Peptide Binding of Escherichia coli DsbC." The Journal of Biological Chemistry. vol. 275, No. 30, pp. 22743-22749. cited by other .
Hsu, Wei-Cherng, Mark H. Spilker, Ioannis V. Yannas, and Peter A. D. Rubin. Aug. 2000. "Inhibition of Conjunctival Scarring and Contraction by a Porous Collagen-Glycosaminoglycan Implant." Investigative Ophthalmology & Visual Science. vol. 41, No. 9, pp. 2404-2411. cited by other .
Schlessinger, Joseph, Alexander N. Plotnikov, Omar A. Ibrahimi, Anna V. Eliseenkova, Brian K. Yeh, Avner Yayon, Robert J. Linhardt, and Moosa Mohammadi. Sep. 2000. "Crystal Structure of a Ternary FGF-FGFR-Heparin Complex Reveals a Dual Role for Heparin in FGFR Binding and Dimerization." Molecular Cell. vol. 6, pp. 743-750. cited by other .
Sun, Y., J. B. Ketterson, G. K. L. Wong. Oct. 9, 2000. "Excitonic Gain and Stimulated Ultraviolet Emission in Nanocrystalline Zinc-Oxide Powder." Applied Physics Letters. vol. 77, No. 15, pp. 2322-2324. cited by other .
Schuldiner, Maya, Ofra Yanuka, Joseph Itskovitz-Eldor, Douglas A. Melton, and Nissim Benvenisty. Oct. 10, 2000. "Effects of Eight Growth Factors on the Differentiation of Cells Derived from Human Embryonic Stem Cells." Proceedings of the National Academy of Sciences of the United States of America. vol. 97, No. 21, pp. 11307-11312. cited by other .
Altman, Michael, Peter Lee, Alexander Rich, and Shuguang Zhang. 2000. "Conformational Behavior of Ionic Self-Complementary Peptides." Protein Science. vol. 9, pp. 1095-1105. cited by other .
Archer, Eric A., Noah T. Goldberg, Vincent Lynch, and Michael J. Krische. 2000. "Nanostructured Polymer Duplexes via the Covalent Casting of 1-Dimensional H-Bonding Motifs: A New Strategy for the Self-Assembly of Macromolecular Precursors." Journal of the American Chemical Society. vol. 122, No. 20, pp. 5006-5007. cited by other .
Ariga, Katsuhiko, Jun-ichi, Masanobu Naito, Emiko Koyama, and Norihiro Yamada. 2000. "Modulated Supramolecular Assemblies Composed of Tripeptide Derivatives: Formation of Micrometer-Scale Rods, Nanometer-Size Needles, and Regular Patterns with Molecular-Level Flatness from the Same Compound." Langmuir. vol. 16, No. 11, pp. 4929-4939. cited by other .
Beniash, E., W. Traub, A. Veis, and S. Weiner. 2000. "A Transmission Electron Microscope Study Using Vitrified Ice Sections of Predentin: Structural Changes in the Dentin Collagenous Matrix Prior to Mineralization." Journal of Structural Biology. vol. 132, pp. 212-225. cited by other .
Bigi, Adriana, Elisa Boanini, Silvia Panzavolta, and Norberti Roveri. 2000. "Biomimetic Growth of Hydroxyapatite on Gelatin Films Doped with Sodium Polyacrylate." Biomacromolecules. vol. 1, No. 4, pp. 752-756. cited by other .
Bourel, Line, Olivier Carion, Helene Gras-Masse, and Oleg Melnyk. 2000. "The Deprotection of Lys(Mtt) Revisited." Journal of Peptide Science. vol. 6, pp. 264-270. cited by other .
Caplan, Michael R., Peter N. Moore, Shuguang Zhang, Roger D. Kamm, and Douglas A. Lauffenburger. 2000. "Self-Assembly of a .beta.-Sheet Protein Governed by Relief of Electrostatic Repulsion Relative to van der Waals Attraction." Biomacromolecules. vol. 1, No. 4, pp. 627-631. cited by other .
Cardullo, F., M. Crego Calama, B. H. M. Snellink-Ruel, J.-L. Weidmann, A. Bielejewska, R. Fokkens, N. M. M. Nibbering, P. Timmerman, and D. N. Reinhoudt. 2000. "Covalent Capture of Dynamic Hydrogen-Bonded Assemblies." Chem. Commun. pp. 367-368. cited by other .
Chamberlain, L. J., I. V. Yannas, H-P. Hsu, G. R. Strichartz, and M. Spector. 2000. "Near-Terminus Axonal Structure and Function Following Rat Sciatic Nerve Regeneration Through a Collagen-GAG Matrix in a Ten-Millimeter Gap." Journal of Neuroscience Research. vol. 60, pp. 666-677. cited by other .
David, Sunil A., Satish K. Awasthi, and P. Balaram. 2000. "The Role of Polar and Facial Amphiphatic Character in Determining Lipopolysaccharide-Binding Properties in Synthetic Cationic Peptides." Journal of Endotoxin Research. vol. 6, No. 3, pp. 249-256. cited by other .
Dori, Yoav, Havazelet Bianco-Peled, Sushil K. Satija, Gregg B. Fields, James B. McCarthy, and Matthew Tirrell. 2000. "Ligand Accessibility as Means to Control Cell Response to Bioactive Bilayer Membranes." Journal of Biomedical Materials Research. vol. 50, pp. 75-81. cited by other .
Forns, Pilar, Janelle L. Lauer-Fields, Su Gao, and Gregg B. Fields. 2000. "Induction of Protein-Like Molecular Architecture by Monoalkyl Hydrocarbon Chains." Biopolymers. vol. 54, pp. 531-546. cited by other .
Hisaeda, Yoshio, Eiji Ohshima, and Makiko Arimura. 2000. "Aggregation Behavior of Synthetic Peptide Lipids with Arginine in Aqueous Solution and Construction of a Vitamin B.sub.12 Artificial Enzyme." Colloids and Surfaces A: Physicochemical and Engineering Aspects. vol. 169, pp. 143-153. cited by other .
Kogiso, Masaki, Yuji Okada, Takeshi Hanada, Kiyoshi Yase, and Toshimi Shimizu. 2000. "Self-Assembled Peptide Fibers from Valylvaline Bola-Amphiphiles by a Parallel .beta.-Sheet Network." Biochimica et Biophysica Acta. vol. 1475, pp. 346-352. cited by other .
Langer, Robert. 2000. "Biomaterials in Drug Delivery and Tissue Engineering: One Laboratory's Experience. " Accounts of Chemical Research. vol. 33, No. 2, pp. 94-101. cited by other .
Liu, X. D., M. Skold, T. Umino, Y. K. Zhu, D.J. Romberger, J. R. Spurzem, and S. I. Rennard. 2000. "Endothelial Cell-Mediated Type I Collagen Gel Contraction Is Regulated by Hemin." J. Lab. Clin. Med. vol. 136, No. 2, pp. 100-109. cited by other .
Lu, Lichun, Susan J. Peter, Michelle D. Lyman, Hui-Lin Lai, Susan M. Leite, Janet A. Tamada, Shiro Uyama, Joseph P. Vacanti, Robert Langer, and Antonios G. Mikos. 2000. "In Vitro and in Vivo Degradation of Porous Poly(DL-Lactic-co-Glycolic Acid) Foams." Biomaterials. vol. 21, pp. 1837-1845. cited by other .
Matsuura, T., R. Hosokawa, K. Okamoto, T. Kimoto, and Y. Akagawa. 2000. "Diverse Mechanisms of Osteoblast Spreading on Hydroxyapatite and Titanium." Biomaterials. vol. 21, pp. 1121-1127. cited by other .
Ponticiello, Michael S., Robert M. Schinagl, Sudha Kadiyala, and Frank P. Barry. 2000. "Gelatin-Based Resorbable Sponge as a Carrier Matrix for Human Mesenchymal Stem Cells in Cartilage Regeneration Therapy." Journal of Biomedical Materials Research. vol. 52, pp. 246-255. cited by other .
Powell, Sharon K., Jayashree Rao, Eva Roque, Motoyoshi Nomizu, Yuichiro Kuratomi, Yoshihiko Yamada, and Hynda K. Kleinman. 2000. "Neural Cell Response to Multiple Novel Sites on Laminin-1." Journal of Neuroscience Research. vol. 61, pp. 302-312. cited by other .
Sakiyama-Elbert, Shelly E. and Jeffrey A. Hubbell. 2000. "Controlled Release of Nerve Growth Factor from a Heparin-Containing Fibrin-Based Cell Ingrowth Matrix." Journal of Controlled Release. vol. 69, pp. 149-158. cited by other .
Sakiyama-Elbert, Shelly E. and Jeffrey A. Hubbell. 2000. "Development of Fibrin Derivatives for Controlled Release of Heparin-Binding Growth Factors." Journal of Controlled Release. vol. 65, pp. 389-402. cited by other .
Thareja, R. K. and A. Mitra. 2000. "Random Laser Action in ZnO." Appl. Phys. vol. B 71, pp. 181-184. cited by other .
Tunggal, Patrick, Neil Smyth, Mats Paulsson, and Mark-Christoph Ott. 2000. "Lamins: Structure and Genetic Regulation." Microscopy Research and Technique. vol. 51, pp. 214-227. cited by other .
do Serro, Ana Paula Valagao Amadeu, Anabela Catarino Fernandes, and Benilde de Jesus Vieira Saramago. 2000. "Calcium Phosphate Deposition on Titanium Surfaces in the Presence of Fibronectin." Journal of Biomedical Materials Research. vol. 49, pp. 345-352. cited by other .
Yamada, Norihiro and Katsuhiko Ariga. 2000. "Formation of .beta.-Sheet Assemblage with a View to Developing an Amyloid Model." Synlett. vol. 5, pp. 575-586. cited by other .
Yu, Huanran, Hiroshi Narusawa, Kisae Itoh. Akihiro Oshi, Narutoshi Yoshino, Kazuo Ohbu, Toshiaki Shirakawa, Kazuhiro Fukada, Masatoshi Fujii, Tadashi Kato, and Tsutomu Semiya. 2000. "Hydrophilicity of Polar and Apolar Domains of Amphiphiles." Journal of Colloid and Interface Science. vol. 229, pp. 375-390. cited by other .
Zhu, G., M. F. Mehler, P. C. Mabie, and J. A. Kessler. 2000. "Developmental Changes in Neural Progenitor Cell Lineage Commitment Do Not Depend on Epidermal Growth Factor Receptor Signaling." Journal of Neuroscience Research. vol. 59, pp. 312-320. cited by other .
Orlic, Donald, Jan Kajstura, Stefano Chimenti, Igor Jakonuk, Stacie M. Anderson, Baosheng Li, James Pickel, Ronald McKay, Bernardo Nadal-Ginard, David M. Bodine, Annarosa Leri, and Piero Anversa. Apr. 5, 2001. "Bone Marrow Cells Regenerate Infarcted Myocardium." Nature. vol. 410, pp. 701-705. cited by other .
Vailhe, Bruno, Daniel Vittet, and Jean-Jacques Feige. Apr. 2001. "In Vitro Models of Vasculogenesis and Angiogenesis." Laboratory Investigation. vol. 81, No. 4, pp. 439-452. cited by other .
Davis, N. G., J. Teisen, C. Schuh, and D. C. Dunand. May 2001. "Solid-State Foaming of Titanium by Superplastic Expansion of Argon-Filled Pores." J. Mater. Res. vol. 16, No. 5, pp. 1508-1519. cited by other .
Rabchevsky, Alexander G. and George M. Smith. May 2001. "Therapeutic Interventions Following Mammalian Spinal Cord Injury." Arch. Neurol. vol. 58, pp. 721-726. cited by other .
Huang, Michael H., Samuel Mao, Henning Feick, Haoquan Yan, Yiying Wu, Hannes Kind, Eicke Weber, Richard Russo, and Peidong Yang. Jun. 8, 2001. "Room-Temperature Ultraviolet Nanowire Nanolasers." Science. vol. 292, pp. 1897-1899. cited by other .
Lee, Kuen Yong and David J. Mooney. Jul. 2001. "Hydrogels for Tissue Engineering." Chemical Reviews. vol. 101, No. 7, pp. 1869-1879. cited by other .
Aggeli, A., I. A. Nyrkova, M. Bell, R. Harding, L. Carrick, T. C. B. McLeish, A. N. Semenov, and N. Boden. Oct. 9, 2001. "Hierarchical Self-Assembly of Chiral Rod-Like Molecules as a Model for Peptide .beta.-Sheet Tapes, Ribbons, Fibrils, and Fibers." Proceedings of the National Academy of Sciences of the United States of America. vol. 98, No. 21, pp. 11857-11862. cited by other .
Richardson, Thomas P., Martin C. Peters, Alessandra B. Ennett, and David J. Mooney. Nov. 2001. "Polymeric System for Dual Growth Factor Delivery." Nature Biotechnology. vol. 19, pp. 1029-1034. cited by other .
Mathew, Mathai and Shozo Takagi. Nov. -Dec. 2001. "Structures of Biological Minerals in Dental Research." Journal of Research of the National Institute of Standards and Technology. vol. 106, No. 6, pp. 1035-1044. cited by other .
Woo, Byung Ho, Betsy F. Fink, Richard Page, Jay A. Schrier, Yeong Woo Jo, Ge Jiang, Michelle DeLuca, Henry C. Vasconez, and Patrick P. DeLuca. Dec. 2001. "Enhancement of Bone Growth by Sustained Delivery of Recombinant Human Bone Morphogenetic Protein-2 in a Polymeric Matrix." Pharmaceutical Research. vol. 18, No. 12, pp. 1747-1753. cited by other .
Barrere, F., P. Layrolle, C. A. Van Blitterswijk, and K. de Groot. 2001. "Biomimetic Coatings on Titanium: A Crystal Growth Study of Octacalcium Phosphate." Journal of Materials Science: Materials in Medicine. vol. 12, pp. 529-534. cited by other .
Bianco-Peled, Havazelet, Yoav Dori, James Schneider, Li-Piin Sung, Sushil Satija, and Matthew Tirrell. 2001. "Structural Study of Langmuir Monolayers Containing Lipidated Poly(ethylene glycol) and Peptides." Langmuir. vol. 17, No. 22, pp. 6931-6937. cited by other .
Cavalli, M., G. Gnappi, A. Montenero, D. Bersani, P. P. Lottici, S. Kaciulis, G. Mattogno, and M. Fini. 2001. "Hydroxy- and Fluorapatite Films on Ti Alloy Substrates: Sol-gel Preparation and Characterization." Journal of Materials Science. vol. 36, pp. 3253-3260. cited by other .
Chang, John C., Gregory J. Brewer, and Bruce C. Wheeler. 2001. "Modulation of Neural Network Activity by Patterning. " Biosensors & Bioelectronics. vol. 16, pp. 527-533. cited by other .
Chang, Sophia C. N., Jon A. Rowley, Geoffrey Tobias, Nicholas G. Genes, Amit K. Roy, David J. Mooney, Charles A. Vacanti, Lawrence J. Bonassar. 2001. "Injection Molding of Chondrocyte/Alginate Constructs in the Shape of Facial Implants." Journal of Biomedical Materials Research. vol. 55, pp. 503-511. cited by other .
Doi, Tomokiyo, Takatoshi Kinoshita, Hiroki Kamiya, Shintaro Washizu, Yoshiharu Tsujita, and Hiraoki Yoshimizu. 2001. "Aggregation of Polypeptide-Based Amphiphiles in Water." Polymer Journal. vol. 33, No. 2, pp. 160-164. cited by other .
Gore, Tushar, Yoav Dori, Yeshayahu Talmon, Matthew Tirrell, and Havazelet Bianco-Peled. 2001. "Self-Assembly of Model Collagen Peptide Amphiphiles." Langmuir. vol. 17, No. 17, pp. 5352-5360. cited by other .
Hoess, Ronald H. 2001. "Protein Design and Phage Display." Chemical Reviews. vol. 101, No. 10, pp. 3205-3218. cited by other .
Huang, Eric J. and Louis F. Reichardt. 2001. "Neurotrophins: Roles in Neuronal Development and Function." Annual Review of Neuroscience. vol. 24, pp. 677-736. cited by other .
Kam, L., W. Shain, J. N. Turner, and R. Bizios. 2001. "Axonal Outgrowth of Hippocampal Neurons on Micro-Scale Networks of Polylysine-Conjugated Laminin." Biomaterials. vol. 22, pp. 1049-1054. cited by other .
Kikuchi, Masanori, Soichiro Itoh, Shizuko Ichinose, Kenichi Shinomiya, and Junzo Tanaka. 2001. "Self-Organization Mechanism in a Bone-Like Hydroxyapatite/Collagen Nancomposite Synthesized in Vitro and Its Biological Reaction in Vivo." Biomaterials. vol. 22, pp. 1705-1711. cited by other .
Liu, Yuelian, Pierre Layrolle, Joost de Bruijn, Clemens van Blitterswijk, and Klaas de Groot. 2001. "Biomimetic Coprecipitation of Calcium Phosphate and Bovine Serum Albumin on Titanium Alloy." Journal of Biomedical Materials Research. vol. 57, pp. 327-335. cited by other .
Look, D. C. 2001. "Recent Advances in ZnO Materials and Devices." Materials Science and Engineering. vol. B80, pp. 383-387. cited by other .
Matsui, Hiroshi, and Gary E. Douberly. Jr. 2001. "Organization of Peptide Nanotubes into Macroscopic Bundles." Langmuir. vol. 17, No. 25, pp. 7918-7922. cited by other .
Neet, K. E. and R. B. Campenot. 2001. "Receptor Binding, Internalization, and Retrograde Transport of Neurotrophic Factors." CMLS, Cell Mol. Life Sci. vol. 58, pp. 1021-1035. cited by other .
Otsuka, Hidenori, Yukio Nagasaki, and Kazunori Kataoka. 2001. "Self-Assembly of Poly(ethylene glycol)--based Block Copolymers for Biomedical Applications." Current Opinion in Colloid & Interface Science. vol. 6, pp. 3-10. cited by other .
Shimizu, Toshimi, Riki Iwaura, Mitsutoshi Masuda, Takeshi Hanada, and Kiyoshi Yase. 2001. "Internucleobase-Interaction-Directed Self-Assembly of Nanofibers from Homo- and Heteroditopic 1,.omega.-Nucleobase Bolaamphiphiles." Journal of the American Chemical Society. vol. 123, No. 25, pp. 5947-5955, S1-S16. cited by other .
Socrates, George. 2001. Infrared and Raman Characteristic Group Frequencies: Tables and Charts. Third Edition. Chichester, England: John Wiley & Sons Ltd. cited by other .
Spanos, Nikos and Petros G. Koutsoukos. 2001. "Model Studies of the Effect of Orthophospho-L-Serine on Biological Mineralization." Langmuir. vol. 17, No. 3, pp. 866-872. cited by other .
Takadama, Hiroaki, Hyun-Min Kim, Tadashi Kokubo, and Takashi Nakamura. 2001. "TEM-EDX Study of Mechanism of Bonelike Apatite Formation on Bioactive Titanium Metal in Simulated Body Fluid." Journal of Biomedical Materials Research. vol. 57, pp. 441-448. cited by other .
Tanihara, Masao, Yasuo Suzuki, Eriko Yamamoto, Atsushi Noguchi, and Yutaka Mizushima. 2001. "Sustained Release of Basic Fibroblast Growth Factor and Angiogenesis in a Novel Covalently Crosslinked Gel of Heparin and Alginate." Journal of Biomedical Materials Research. vol. 56, pp. 216-221. cited by other .
Torchilin, Vladimir P. 2001. "Structure and Design of Polymeric Surfactant-Based Drug Delivery Systems." Journal of Controlled Release. vol. 73, pp. 137-172. cited by other .
Yeung, C. K., L. Lauer, A. Offenhausser, and W. Knoll. 2001. "Modulation of the Growth and Guidance of Rat Brain Stem Neurons Using Patterned Extracellular Matrix Proteins." Neuroscience Letters. vol. 301, pp. 147-150. cited by other .
Zubarev, Eugene R., Martin U. Pralle, Eli D. Stone, and Samuel I. Stupp. 2001. "Self-Assembly of Dendron Rodcoil Molecules into Nanoribbons." Journal of the American Chemical Society. vol. 123, No. 17, pp. 4105-4106. cited by other .
Hirschi, Karen K., Lihua Lai, Narasimhaswamy S. Belaguli, David A. Dean, Robert J. Schwartz, and Warren E. Zimmer. Feb. 22, 2002. "Transforming Growth Factor-.beta. Induction of Smooth Muscle Cell Phenotype Requires Transcriptional and Post-transcriptional Control of Serum Response Factor." The Journal of Biological Chemistry. vol. 277, No. 8, pp. 6287-6295. cited by other .
Xu, Weiming, Lizhi Liu, and Ian G. Charles. Feb. 2002. "Microencapsulated iNOS-expressing Cells Cause Tumor Suppression in Mice." The FASEB Journal. vol. 16, pp. 213-215. cited by other .
Zubarev, Eugene R., Martin U. Pralle, Eli D. Sone, and Samuel I. Stupp. Feb. 2002. "Scaffolding of Polymers by Supramolecular Nanoribbons." Advanced Materials. vol. 14, No. 3, pp. 198-203. cited by other .
Teng, Yang D., Erin B. Lavik, Xianlu Qu, Kook I. Park, Jitka Ourednik, David Zurakowski, Robert Langer, and Evan Y. Snyder. Mar. 5, 2002. "Functional Recovery Following Traumatic Spinal Cord Injury Mediated by a Unique Polymer Scaffold Seeded with Neural Stem Cells." Proceedings of the National Academy of Sciences of the United States of America. vol. 99, No. 5, pp. 3024-3029. cited by other .
Bradbury, Elizabeth J., Lawrence D. F. Moon, Reena J. Popat, Von R. King, Gavin S. Bennett, Preena N. Patel, James W. Fawcett, and Stephen B. McMahon. Apr. 11, 2002. "Chondroitinase ABC Promotes Functional Recovery After Spinal Cord Injury." Nature. vol. 416, pp. 636-640. cited by other .
Vauthey, Sylvain, Steve Santoso, Haiyan Gong, Nicki Watson, and Shuguang Zhang. Apr. 16, 2002. "Molecular Self-Assembly of Surfactant-like Peptides to Form Nanotubes and Nanovesicles." Proceedings of the National Academy of Sciences of the United States of America. vol. 99, No. 8, pp. 5355-5360. cited by other .
Nowak, Andrew P., Victor Breedveld, Lisa Pakstis, Bulent Ozbas, David J. Pine, Darrin Pochan, and Timothy J. Deming. May 23, 2002. "Rapidly Recovering Hydrogel Scaffolds from Self-Assembling Diblock Copolypeptide Amphiphiles." Nature. vol. 417, pp. 424-428. cited by other .
GrandPre, Tadzia, Shuxin Li, and Stephen M. Strittmatter. May 30, 2002. "Nogo-66 Receptor Antagonist Peptide Promotes Axonal Regeneration." Nature. vol. 417, pp. 547-551. cited by other .
Storch, Alexander and Johannes Schwarz. May 2002. "Neural Stem Cells and Neurodegeneration." Current Opinion in Investigational Drugs.vol. 3, No. 5, pp. 774-781. cited by other .
Lendlein, Andreas and Robert Langer. May 31, 2002. "Biodegradable, Elastic Shape-Memory Polymers for Potential Biomedical Applications." Science. vol. 296, pp. 1673-1676. cited by other .
Qiu, Jin, Dongming Cai, Haining Dai, Marietta McAttee, Paul N. Hoffman, Barbara S. Bregman, and Marie T. Filbin. Jun. 13, 2002. "Spinal Axon Regeneration Induced by Elevation of Cyclic AMP." Neuron. vol. 34, pp. 895-903. cited by other .
Catledge, Shane A., Marc D. Fries, Yogesh K. Vohra, William R. Lacefield, Jack E. Lemons, Shanna Woodard, and Ramakrishna Venugopalan. Jun-Aug. 2002. "Nanostructured Ceramics for Biomedical Implants." Journal of Nanoscience and Nanotechnology. vol. 2, No. 3/4, pp. 293-312. cited by other .
Alsberg, Eben, Kenneth W. Anderson, Amru Albeiruti, Jon A. Rowley, and David J. Mooney. Sep. 17, 2002. "Engineering Growing Tissues." Proceedings of the National Academy of Sciences of the United States of America. vol. 99, No. 19, pp. 12025-12030. cited by other .
Kay, Sarina, Anil Thapa, Karen M. Haberstroh, and Thomas J. Webster. Oct. 2002. "Naostructured Polymer/Nanophase Ceramic Composites Enhance Osteoblast and Chondrocyte Adhesion." Tissue Engineering. vol. 8, No. 5, pp. 753-761. cited by other .
Chang, Hua, Chester W. Brown, and Martin M. Matzuk. Dec. 2002. "Genetic Analysis of the Mammalian Transforming Growth Factor-.beta. Superfamily." Endocrine Reviews. vol. 23, No. 6, pp. 787-823. cited by other .
Busque, Felix, Stephanie A. Hopkins, and Joseph P. Konopelski. 2002. "Progress Toward a Peptidomimetic of Laminin-Derived Pentapeptide YIGSR: Synthesis of the Unique Tricyclic Core Structure." J. Org. Chem. vol. 67, No. 17, pp. 6097-6103. cited by other .
Canaple, Laurence, Annemie Rehor, and David Hunkeler. 2002. "Improving Cell Encapsulation Through Size Control." J. Biomater. Sci. Polymer Edn. vol. 13, No. 7, pp. 783-796. cited by other .
Caplan, Michael R., Elissa M. Schwartzfarb, Shuguang Zhang, Roger D. Kamm, and Douglas A. Lauffenburger. 2002. "Control of Self-Assembling Oligopeptide Matrix Formation Through Systematic Variation of Amino Acid Sequence." Biomaterials. vol. 23, pp. 219-227. cited by other .
Chen, Zhi Jiang, Yvonne Ughrin, and Joel M. Levine. 2002. "Inhibition of Axon Growth by Oligodendrocyte Precursor Cells." Molecular and Cellular Neuroscience. vol. 20, pp. 125-139. cited by other .
Cornish, Toby, Darren W. Branch, Bruce C. Wheeler, and James T. Campanelli. 2002. "Microcontact Printing: A Versatile Technique for the Study of Synaptogenic Molecules." Molecular and Cellular Neuroscience. vol. 20, pp. 140-153. cited by other .
Costa, Silvia, Thierry Planchenault, Cecile Charriere-Bertrand, Yann Mouchel, Christiane Fages, Sharon Juliano, Thierry Lefrancois, Georgia Barlovatz-Meimon, and Marcienne Tardy. 2002. "Astroglial Permissivity for Neuritic Outgrowth in Neuritic Outgrowth in Neuron-Astrocyte Cocultures Depends on Regulation of Laminin Bioavailabilty." GLIA. vol. 37, pp. 105-113. cited by other .
Gariepy Jean, Sandrine Remy, Xiuguo Zhang, James R. Ballinger, Eleonora Bolewska-Pedyczak, Michael Rauth, and Stuart K. Bisland. 2002. "A Simple Two-Step Approach for Introducing a Protected Diaminedithiol Chelator During Solid-Phase Assembly of Peptides." Bioconjugate Chem. vol. 13, No. 3, pp. 679-684. cited by other .
Glattli, Alice, Xavier Daura, Dieter Seebach, and Wilfred F. van Gunsteren. 2002. "Can One Derive the Confrontational Preference of a .beta.-Peptide from Its CD Spectrum?" Journal of the American Chemical Society. vol. 124, No. 44, pp. 12972-12978. cited by other .
Gutwein, Luke G. and Thomas J. Webster. 2002. "Osteoblast and Chondrocyte Proliferation in the Presence of Alumina and Titania Nanoparticles." Journal of Nanoparticle Research. vol. 4, pp. 231-238. cited by other .
Huang, Ning-Ping, Gabor Csucs, Kazunori Emoto, Yukio Nagasaki, Kazunori Kataoka, Marcus Textor, and Nicholas D. Spencer. 2002. "Covalent Attachment of Novel Poly(ethylene glycol)--Poly(DL-lactic acid) Colpolymeric Micelles to TiO.sub.2 Surfaces." Langmuir. vol. 18, No. 1, pp. 252-258. cited by other .
Issac, Roy and Jean Chmielewski. 2002. "Approaching Exponential Growth with a Self-Replicating Peptide." Journal of the American Chemical Society. vol. 124, No. 24, pp. 6808-6809. cited by other .
Joshi, Mital and Michael G. Fehlings. 2002. "Development and Characterization of a Novel, Graded Model of Clip Compressive Spinal Cord Injury in the Mouse: Part 1. Clip Design, Behavioral Outcomes, and Histopathology." Journal of Neurotrauma. vol. 19, No. 2, pp. 175-190. cited by other .
Joshi, Mital and Michael G. Fehlings. 2002. "Development and Characterization of a Novel, Graded Model of Clip Compressive Spinal Cord Injury in the Mouse: Part 2. Quantitative Neuroanatomical Assessment and Analysis of the Relationships Between Axonal Tracts, Residual Tissue, and Locomotor Recovery." Journal of Neurotrauma. vol. 19, No. 2, pp. 191-203. cited by other .
Kruger, Ryan G., Patrick Dostal, and Dewey G. McCafferty. 2002. "An Economical and Preparative Orthogonal Solid Phase Synthesis of Fluorescein and Rhodamine Derivatized Peptides: FRET Substrates for the Staphylococcus aureus Sortase SrtA Transpeptidase Reaction." Chem. Commun. pp. 2092-2093. cited by other .
Lauer, L., A. Vogt, C. K. Yeung, W. Knoll, and A. Offenhausser. 2002. "Electrophysiological Recordings of Patterned Rat Brain Stem Slice Neurons." Biomaterials. vol. 23, pp. 3123-3130. cited by other .
Lavik, Erin, Yang D. Teng, Evan Snyder, and Robert Langer. 2002. "Speeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers." Methods in Molecular Biology: Neural Stem Cells: Methods and Protocols. vol. 198, pp. 89-97. cited by other .
Marini, Davide M., Wonmuk Hwang, Douglas A. Lauffenburger, Shuguang Zhang, and Roger D. Kamm. 2002. "Left-Handed Helical Ribbon Intermediates in the Self-Assembly of a .beta.-Sheet Peptide." Nao Letters. vol. 2, No. 4, pp. 295-299. cited by other .
Ohsaki, Mio, Tatsuya Okuda, Akihiro Wada, Toshiya Hirayama, Takuo Niidome, and Haruhiko Aoyagi. 2002. "In Vitro Gene Transfection Using Dendritic Poly(L-lysine)." Bioconjugate Chem. vol. 13, No. 3, pp. 510-517. cited by other .
Okano, Hideyuki. 2002. "Stem Cell Biology of the Central Nervous System." Journal of Neuroscience Research. vol. 69, pp. 698-707. cited by other .
Parmar, Malin, Charlotta Skogh, Anders Bjorklund, and Kenneth Campbell. 2002. "Regional Specification of Neurosphere Cultures Derived from Subregions of the Embryonic Telencephalon." Molecular and Cellular Neuroscience. vol. 21, pp. 645-656. cited by other .
Porter, A. E., L. W. Hobbs, V. Benezra Rosen, and M. Spector. 2002. "The Ultrastructure of the Plasma-Sprayed Hydroxyapatite-bone Interface Predisposing to Bone Bonding." Biomaterials. vol. 23, pp. 725-733. cited by other .
Rowley, Jon A. and David J. Mooney. 2002. "Alginate Type and RGD Density Control Myoblast Phenotype." Journal of Biomedical Materials Research. vol. 60, pp. 217-223. cited by other .
Santoso, Steve S., Sylvain Vauthey, Shuguang Zhang. 2002. "Structures, Function and Applications of Amphiphilic Peptides." Current Opinion of Colloid & Interface Science. vol. 7, pp. 262-266. cited by other .
Thiebaud, Pierre, Lars Lauer, Wolfgang Knoll, and Andreas Offenhauser. 2002. "PDMS Device for Patterned Application of Microfluids to Neuronal Cells Arranged by Microcontact Printing." Biosensors & Bioelectronics. vol. 17, pp. 87-93. cited by other .
Tryoen-Toth, Dominique Vautier, Youssef Haikel, Jean-Claude Voegel, Pierre Schaaf, Johanna Chluba, and Joelle Ogier. 2002. "Viability, Adhesion, and Bone Phenotype of Osteoblast-like Cells on Polyelectrolyte Multilayer Films." Journal of Biomedical Materials Research. vol. 60, pp. 657-667. cited by other .
Young, Wise. 2002. "Spinal Cord Contusion Models." Progress in Brain Research. vol. 137, pp. 231-255. cited by other .
Lutolf, Matthias P., Franz E. Weber, Hugo G. Schmoekel, Jason C. Schense, Thomas Kohler, Ralph Muller, and Jeffey A. Hubbell. May 2003. "Repair of Bone Defects Using Synthetic Mimetics of Collagenous Extracellular Matrices." Nature Biotechnology. vol. 21, pp. 513-518. cited by other .
Shaw, Derek and Molly S. Shoichet. May 2003. "Toward Spinal Cord Injury Repair Strategies: Peptide Surface Modification of Expanded Poly(Tetrafluoroethylene) Fibers for Guided Neurite Outgrowth in Vitro." The Journal of Craniofacial Surgery. vol. 14, No. 3, pp. 308-316. cited by other .
Cheng, Hongwei, Wei Jiang, Frank M. Phillips, Rex C. Haydon, Ying Peng, Lan Zhou, Hue H. Luu, Naili An, Benjamin Breyer, Pantila Vanichakarn, Jan Paul Szatkowski, Jae Yoon Park, and Tong-Chuan He. Aug. 2003. "Osteogenic Activity of the Fourteen Types of Human Bone Morphogenetic Proteins (BMPs)." The Journal of Bone & Joint Surgery. vol. 85-A, No. 8, pp. 1544-1552, 141. cited by other .
Pavlov, Georges, Stephanie Finet, Karine Tatarenko, Evgueniya Korneeva, and Christine Ebel. 2003. "Conformation of Heparin Studied with Macromolecular Hydrodynamic Methods and X-ray Scattering." Eur. Biophys. J. vol. 32, pp. 437-449. cited by other .
Arinzeh, Treena Livingston, Susan J. Peter, Michael P. Archambault, Christian van den Bos, Stevev Gordon, Karl Kraus, Alan Smith, and Sudha Kadiyala. Oct. 2003. "Allogeneic Mesenchymal Stem Cells Regenerate Bone in a Critical-Sized Canine Segmental Defect." The Journal of Bone & Joint Surgery. vol. 85-A, No. 10, pp. 1927-1935. cited by other .
Zhang, Shuguang. Oct. 2003. "Fabrication of Novel Biomaterials Through Molecular Self-Assembly." Nature Biotechnology. vol. 21, No. 10, pp. 1171-1178. cited by other .
Aggeli, Amalia, Mark Bell, Lisa M. Carrick, Colin W. G. Fishwick, Richard Harding, Peter J. Mawer, Sheena E. Radford, Andrew E. Strong, and Neville Boden. 2003. "pH as a Trigger of Peptide .beta.-Sheet Self-Assembly and Reversible Switching Between Nematic and Isotropic Phases." Journal of the American Chemical Society. vol. 125, No. 32, pp. 9619-9628. cited by other .
Alsina, Jordi and Fernando Albericio. 2003. "Solid-Phase Synthesis of C-Terminal Modified Peptides." Biopolymers (Peptide Science). vol. 71, pp. 454-477. cited by other .
Anthony, Shawn G. 2003. "Injectable Biomaterials for Bone Tissue Engineering." cited by other .
Boontheekul, Tanyarut and David J. Mooney. 2003. "Protein-Based Signaling Systems in Tissue Engineering." Current Opinion in Biotechnology. vol. 14, pp. 559-565. cited by other .
Fauza, Dario O. 2003. "Tissue Engineering: Current State of Clinical Application." Current Opinion in Pediatrics. vol. 15, pp. 267-271. cited by other .
Ganesh, S. and R. Jayakumar. 2003. "Structural Transitions Involved in a Novel Amyloid-Like .beta.-Sheet Assemblage of Tripeptide Derivatives." Biopolymers. vol. 70, pp. 336-345. cited by other .
Ganesh, S., S. Prakash, and R. Jayakumar. 2003. "Spectroscopic Investigation on Gel-Forming .beta.-Sheet Assemblage of Peptide Derivatives." Biopolymers. vol. 70, pp. 346-354. cited by other .
Gergely, C. S., P. Bar Yosef, R. Govrin-Lippman, F. Cuisinier, and H. Furedi-Milhofer. 2003 ."The Deposition of Calcium Phosphates Within Polyelectrolyte Multilayer Films." Key Engineering Materials. vols. 240-242 (Bioceramics), pp. 287-290. cited by other .
Goeden-Wood, Nichole L., Jay D. Keasling, and Susan J. Muller. 2003. "Self-Assembly of a Designed Protein Polymer into .beta.-Sheet Fibrils and Responsive Gels." Macromolecules. vol. 36, No. 8, pp. 2932-2938. cited by other .
Ishihara, Masayuki, Kiyohaya Obara, Toshiaki Ishizuka, Masanori Fujita, Masato Sato, Kazunori Masuoka, Yoshio Saito, Hirofumi Yura, Takemi Matsui, Hidemi Hattori, Makoto Kikuchi, and Akira Kurita. 2003. "Controlled Release of Fibroblast Growth Factors and Heparin from Photocrosslinked Chitosan Hydrogels and Subsequent Effect on in Vivo Vascularization." Journal of Biomedical Materials Research. vol. 64A, pp. 551-559. cited by other .
Malkar, Navdeep B., Janelle l. Lauer-Fields, Darius Juska, and Gregg B. Fields. 2003. "Characterization of Peptide-Amphiphiles Possessing Cellular Activation Sequences." Biomacromolecles. vol. 4, No. 3, pp. 518-528. cited by other .
Niece, Krista L., Jeffrey D. Hartgerink, Jack J. J. M. Donners, and Samuel I. Stupp. 2003. "Self-Assembly Combining Two Bioactive Peptide-Amphiphile Molecules into Nanofibers by Electrostatic Attraction. " Journal of the American Chemical Society. vol. 125, No. 24, pp. 7146-7147. cited by other .
Steward, Oswald, Binhai Zheng, and Marc Tessier-Lavigne. 2003. "False Resurrections: Distinguishing Regenerated from Spared Axons in the Injured Central Nervous System." The Journal of Comparative Neurology. vol. 459, pp. 1-8. cited by other .
Wu, Sufan, Yoshihisa Suzuki , Yoko Ejiri, Toru Noda, Hongliang Bai, Masaaki Kitada, Kazuya Kataoka. Masayoshi Ohta, Hirotomi Chou, and Chizuka Ide. 2003. "Bone Marrow Stromal Cells Enhance Differentiation of Cocultured Neurosphere Cells and Promote Regeneration of Injured Spinal Cord." Journal of Neuroscience Research. vol. 72, pp. 343-351. cited by other .
Yamada, Norihiro, Tsukasa Komatsu, Hirotsugu Yoshinaga, Kayo Yoshizawa, Susumu Edo, and Masashi Kunitake. 2003. "Self-Supporting Elastic Film without Covalent Linkages as a Hierarchically Integrated .beta.-Sheet Assembly." Angew. Chem. Int. Ed. vol. 42, pp. 5496-5499. cited by other .
Zhang, Yan, Hongwei Gu, Zhimou Yang, and Bing Xu. 2003. "Supramolecular Hydrogels Respond to Ligand-Receptor Interaction." Journal of the American Chemical Society. vol. 125, No. 45, pp. 13680-13681. cited by other .
Hirano, Yoshiaki and David J. Mooney. Jan. 5, 2004. "Peptide and Protein Presenting Materials for Tissue Engineering." Advanced Materials. vol. 16, No. 1, pp. 17-25. cited by other .
Silva, Gabriel A., Catherine Czeisler, Krista L. Niece, Elia Beniash, Daniel A. Harrington, John A. Kessler, and Samuel I. Stupp. Feb. 27, 2004. "Selective Differentiation of Neural Progenitor Cells by High-Epitope Density Nanofibers." Science. vol. 303, pp. 1352-1355. cited by other .
Faulkner, Jill R., Julia E. Herrmann, Michael J. Woo, Keith E. Tansey, Ngan B. Doan, and Michael V. Sofroniew. Mar. 3, 2004. "Reactive Astrocytes Protect Tissue and Preserve Function after Spinal Cord Injury." The Journal of Neuroscience. vol. 24, No. 9, pp. 2143-2155. cited by other .
Cao, Renhai, Anna Eriksson, Hajime Kubo, Kari Alitalo, Yihai Cao, Johan Thyberg. Mar. 19, 2004. "Comparative Evaluation of FGF-2-, VEGF-A-, and VEGF-C-Induced Angiogenesis, Lymphangiogenesis, Vascular Fenestrations, and Permeability." Circulation Research. vol. 94, pp. 664-670. cited by other .
Anthony, Shawn G. Mar. 28-Apr. 1, 2004. "Self-Assembling Nanofiber Matrix for Bone Regeneration." The 227.sup.th ACS National Meeting. Anaheim, CA. cited by other .
Donners, Jack J. J. M. Mar. 28-Apr. 1, 2004. "Growth Factor Binding Self-Assembling Nanofiber Networks for Tissue Regeneration." The 227.sup.th ACS National Meeting. Anaheim, CA. cited by other .
Nikulina Elena, J. Lille Tidwell, Hai Ning Dai, Barbara S. Bregman, and Marie T. Filbin. Jun. 8, 2004. "The Phosphodiesterase Inhibitor Rolipram Delivered after a Spinal Cord Lesion Promotes Axonal Regeneration and Functional Recovery." Proceedings of the National Academy of Sciences of the United States of America. vol. 101, No. 23, pp. 8786-8790. cited by other .
Pearse, Damien D., Francisco C. Pereira, Alexander E. Marcillo, Margaret L. Bates, Yerko A. Berrocal, Marie T. Filbin, and Mary Bartlett Bunge. Jun. 2004. "cAMP and Schwann Cells Promote Axonal Growth and Functional Recovery After Spinal Cord Injury." Nature Medicine. vol. 10, No. 6, pp. 610-616. cited by other .
Lu, Paul, Hong Yang, Leonard L. Jones, Marie T. Filbin, and Mark H. Tuszynski. Jul. 14, 2004. "Combinatorial Therapy with Neurotrophins and cAMP Promotes Axonal Regeneration beyond Sites of Spinal Cord Injury." The Journal of Neuroscience. vol. 24, No. 28, pp. 6402-6409. cited by other .
Lee, K. W., J. J. Yoon, J. H. Lee, S. Y. Kim, H. J. Jung, S. J. Kim, J. W. Joh, H. H. Lee, D. S. Lee, and S. K. Lee. 2004. "Sustained Release of Vascular Endothelial Growth Factor From Calcium-Induced Alginate Hydrogels Reinforced by Heparin and Chitosan." Transplantation Proceedings. vol. 36, pp. 2464-2465. cited by other .
Matsumura, Sachiko, Shinobu Uemura, Hisakazu Mihara. 2004. "Fabrication of Nanofibers with Uniform Morphology by Self-Assembly of Designed Peptides." Chem. Eur. J. vol. 10, pp. 2789-2794. cited by other .
Sieminski, A. L., R. P. Hebbel, and K. J. Gooch. 2004. "The Relative Magnitudes of Endothelial Force Generation and Matrix Stiffness Modulate Capillary Morphogenesis in Vitro. " Experimental Cell Research. vol. 297, pp. 574-584. cited by other .
Vandermeulen, Guido W. M. and Harm-Anton Klok. 2004. "Peptide/Protein Hybrid Materials: Enhanced Control of Structure and Improved Performance through Conformation of Biological and Synthetic Polymers." Macromolecular Bioscience. vol. 4, pp. 383-398. cited by other .
Wang, Lin-Fa and Meng Yu. 2004. "Epitope Identification and Discovery Using Phage Display Libraries: Applications in Vaccine Development and Diagnostics." Current Drug Targets. vol. 5, No. 1, pp. 1-15. cited by other .
Sayle, Roger. Printed Nov. 9, 2005. "Physiological Ionization and pKa Prediction." http://www.daylight.com/meetings/emug00/Sayle/pkapredict.html. pp. 1-13. cited by other .
Bull, Steve R., Mustafa O. Guler, Rafael E. Bras, Thomas J. Meade, and Samuel I. Stupp. 2005. "Self-Assembled Peptide Amphiphile Nanofibers Conjugated to MRI Contrast Agents." Nano Letters. vol. 5, No. 1, pp. 1-4. cited by other .
Guler, Mustafa O., Stephen Soukasene, James F. Hulvat, and Samuel I. Stupp. 2005. "Presentation and Recognition of Biotin on Nanofibers Formed by Branched Peptide Amphiphiles." Nano Letters. vol. 5, No. 2, pp. 249-252. cited by other .
Loudon, M. "Amino Acid Structures at Physiological pH." Printed Jun. 5, 2006. www.brynmawr.edu/Acads/Chem/mnerzsto/amino.sub.--acids.sub.--2.gif, and amino.sub.--acids3.htm. cited by other.

Primary Examiner: Gupta; Anish

Government Interests



The United States government has certain rights to this invention pursuant to Grant Nos. DE-FG02-00ER45810/A001, DMR9996253 and F49620-00-1-0283/P01 from, respectively, the DOE, NSF and AFOSR-MURI to Northwestern University.
Parent Case Text



This application claims priority benefit from provisional patent application Ser. No. 60/333,074 filed Nov. 14, 2001, the entirety of which is incorporated herein by reference.
Claims



What is claimed:

1. A peptide amphiphile composition comprising a peptide amphiphile selected from the group consisting of CCCCGGGSRGD (SEQ ID NO: 2), CCCCGGGSRGDS (SEQ ID NO: 17), CCCCGGGSKGE (SEQ ID NO: 11), CCCCGGGEIKVAV (SEQ ID NO: 16), AAAAGGGSRGD (SEQ ID NO: 9), AAAAGGGSKGE (SEQ ID NO: 12), and GGGSRGD (SEQ ID NO: 7), wherein the serine residue in the peptide amphiphile is optionally phosphorylated and wherein a C.sub.10-C.sub.22 hydrocarbon component is attached to the N-terminus of the peptide component, which directs said composition to form a cylindrical micelle in an aqueous medium.

2. The peptide amphiphile composition of claim 1, wherein said peptide amphiphile is CCCCGGGEIKVAV (SEQ ID NO: 16).

3. The peptide amphiphile composition of claim 1, wherein said peptide amphiphile is AAAAGGGSRGD (SEQ ID NO:2).

4. The peptide amphiphile composition of claim 3, wherein the serine residue of the peptide amphiphile is phosphorylated.

5. A sol-gel system comprising a solvent medium comprising a peptide amphiphile composition of claim 1, and a reagent to adjust system pH.

6. The system of claim 5 wherein said reagent is an acid, said pH is less than about 4, and said system comprises a gel of cylindrical micelles of said amphiphile composition.

7. The system of claim 5 wherein said reagent is a base, said pH is greater than about 4, and said system comprises a solution of said amphiphile composition.

8. The system of claim 7 further comprising an oxidizing agent to form a disulfide bond between amphiphile compositions.

9. The system of claim 8 further comprising a subsequent reducing agent to cleave said disulfide bond.

10. A nanofibrous micelle template for crystal growth, said template comprising an amphiphile composition of claim 1, said peptide component of said composition comprising a residue with a functional moiety capable of intermolecular covalent bond formation.

11. The template of claim 10 comprising a plurality of cysteine residues, wherein the thiol moieties thereof are oxidized to form a disulfide bond between amphiphile compositions.

12. The template of claim 10 wherein said peptide amphiphile component comprises at least one phosphoserine residue.

13. An organo-mineral composite comprising a nanofibrous micelle template of claim 12, and hydroxyapatite crystals on said micelle template, the c-axes of said crystals aligned substantially parallel with the longitudinal axis of said template.
Description



BACKGROUND OF THE INVENTION

Self-assembly and biomineralization are used for fabrication of many composite materials. Bone tissue is a particularly complex example of such a composite because it contains multiple levels of hierarchical organization (S. Weiner, H. D. Wagner, Annu. Rev. Mater. Sci. 28, 271-298 (1998)). At the lowest level of this hierarchy is the organization of collagen fibrils with respect to hydroxyapatite (HA) crystals. Collagen fibrils are formed by self-assembly of collagen triple helices while the HA crystals grow within these fibrils in such a way that their c-axes are oriented along the long axes of the fibrils (W. Traub, S. Weiner, Proc. Nat. Acad. Sci. 86, 9822-9826 (1989)). The preparation of any material with structure on the nanoscale is a challenging problem. Fabrication of materials that resemble bone, even at the lowest level of hierarchical organization, is even more difficult because it involves two dissimilar organic and inorganic nanophases that have a specific spatial relationship with respect to one another. One approach, using an artificial system, has been to prepare an organic nanophase designed to exert control over crystal nucleation and growth of the inorganic component.

The controlled nucleation and growth of crystals from organic templates has been demonstrated in in vitro experiments and in a number of natural biomineralizing systems (S. Mann, J. P. Hannington, R. J. P. Williams, Nature 324, 565-567 (1986); D. D. Archibald, S. Mann, Nature 364, 430-433 (1993); S. L. Burkett, S. Mann, Chem. Commun. 321-322 (1996); S. I. Stupp, P. V. Braun, Science 277, 1242-1248 (1997); J. Aizenberg, A. J. Black, G. M. Whitesides, Nature 398, 495-498 (1999); S. R. Whaley, D. S. English, E. L. Hu, P. F. Barbara, A. M. Belcher, Nature 405, 665-668. (2000); L. Addadi, S. Weiner, Angew. Chem., Int. Ed. Engl. 31, 153-169 (1992); S. Mann, J. Chem. Soc., Dalton Tran. 3953-3961 (1997); S. Weiner, L. Addadi, J. Mater. Chem. 7, 689-702 (1997)). These studies on templated crystal growth suggest that nucleation occurs on surfaces exposing repetitive patterns of anionic groups. Anionic groups tend to concentrate the inorganic cations creating local supersaturation followed by oriented nucleation of the crystal. Many groups have investigated the preparation of bone-like materials using three dimensional organic substrates such as poly(lactic acid), reconstituted collagen and many others, and some studies shows a similar correlation between the crystallographic orientation of hydroxyapatite when the organic scaffold is made from reconstituted collagen(G. K. Hunter, H. A. Goldberg, Biochem. J. 302, 175-179 (1994); G. M. Bond, R. H. Richman, W. P. McNaughton, J. Mater. Eng. Perform. 4, 334-345 (1995); J. -H. Bradt, M. Mertig, A. Teresiak, W. Pompe, Chem. Mater. 11, 2694-2701 (1999); N. Ignjatovic, S. Tomic, M. Dakic, M. Miljkovic, M. Plavsic, D. Uskokovic, Biomaterials 20, 809-816 (1999); F. Miyaji, H. -M. Kim, S. Handa, T. Kokubo, T. Nakamura, Biomaterials 20, 913-919 (1999); H. K. Varma, Y. Yokogawa, F. F. Espinosa, Y. Kawamoto, K. Nishizawa, F. Nagata, T. Kameyama, J. Mater. Sci.: Mater. Med. 10, 395-400 (1999); A. Bigi, E. Boanini, S. Panzavolta, N. Roveri, Biomacromolecules 1, 752-756 (2001); M. Kikuchi, S. Itoh, S. Ichinose, K. Shinomiya, J. Tanaka, Biomaterials 22, 1705-1711 (2001)). However, such results have never been demonstrated in a pre-designed and engineered self-assembling molecular system.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1. In accordance with this invention: a) Chemical structure of a preferred peptide amphiphile, highlighting one or more structural features thereof. Region 1 may comprise a long alkyl tail that conveys hydrophobic character to the molecule and combined with the peptide region makes the molecule amphiphilic. Region 2 may comprise one or more (four consecutive, shown) cysteine residues which when oxidized may form disulfide bonds to polymerize the self-assembled structure. Region 3 may comprise a flexible linker region of one or more glycine residues, preferably three, or functionally similar such residues or monomers, to provide the hydrophilic head group flexibility from the more rigid crosslinked region. Region 4 may comprise a single phosphorylated serine residue which is designed to interact strongly with calcium ions and help direct mineralization of hydroxyapatite. Region 5 may comprise a cell adhesion ligand RGD. b) Molecular model of an illustrated PA showing the overall conical shape of the molecule going from the narrow hydrophobic tail to the bulkier peptide region. c) Schematic showing the self-assembly of PA molecules into a cylindrical micelle.

FIG. 2. a) Negative stain (phosphotungstic acid) TEM of self-assembled nanofibers before covalent capture. Fibers are arranged in ribbon-like parallel arrays. b) Vitreous ice cryo-TEM of the fibers reveals the diameter of the fibers in their native hydrated state to be 7.6.+-.1 nm. c) Positive stain (uranyl acetate) TEM of the self-assembled nanofibers after oxidative cross-linking showing electron dense regions due to the stain that localized on the periphery of the fibers. d) Thin section TEM of positively stained (uranyl acetate) nanofibers after oxidative cross-linking and embedding in epoxy resin. Two fibers are observed in cross-section (arrows) clearly showing the lack of staining in the interior of the fiber.

FIG. 3. a) TEM micrographs of the unstained, cross-linked peptide-amphiphile fibers incubated for 10 min in CaCl.sub.2 and Na.sub.2HPO.sub.4 solution. The fibers arranged in bundles are visible due to the high concentration of inorganic ions on their surface. b) After 20 minutes forming HA crystals (arrows) are observed in parallel arrays on some of the PA fibers. c) After 30 minutes mature HA crystals (arrows) completely cover the PA fibers. d) Electron diffraction pattern taken from a mineralized bundle of PA fibers after 30 minutes of exposure to calcium and phosphate. The presence and orientation of the diffraction arcs corresponding to the 002 and 004 planes indicate preferential alignment of the crystals with their c-axes along the long axis of the bundle. e) Plot of intensity versus inverse angstroms reveals that the 002 and 004 peaks of hydroxyapatite are strongly enhanced along the peptide-amphiphile fiber axis. f) EDS profile of mineral crystals after 30 minutes of incubation reveals a Ca/P ratio of 1.67.+-.0.08 as expected for HA.

FIG. 4. Scheme showing possible relationships between peptide-amphiphile fibers and hydroxyapatite crystals in the mineralized bundle. Arrow indicates the direction of the c-axes of the crystals.

FIG. 5. A tilt pair taken from mineralized PA fibers after 30 minutes of incubation with calcium and phosphate demonstrating the plate shape of the crystals. The crystals that were "edge-on" (electron dense, narrow objects) in the zero degree image lose contrast in the 45 degree rotated image (arrow 1) while the contrast of the crystals that were "face-on" in the zero degree images increase (arrow 2).

FIG. 6. a) Nonphosphorylated PA fibers after 20 minutes of incubation with calcium and phosphate shows only amorphous mineral deposit concentrate on the fibers. b) Nonphosphorylated PA after 30 minutes of incubation with calcium and phosphate continue to show only amorphous mineral in contrast with phosphorylated PA which shows heavy crystallization at this time point. FIGS. 7-9. TEM micrographs for several cylindrical micelles prepared from PA molecules listed in Table 1. Specifically: FIG. 7, Top: Molecule #4 containing a C10 alkyl tail. Bottom: Molecule #13 containing a C22 alkyl tail; FIG. 8, Top: Molecule 8 utilizing a tetra alanine sequence in place of tetra cysteine and containing a C 16 alkyl tail. Bottom: Molecule 9 utilizing a tetra alanine sequence in place of tetra cysteine and containing a C10 alkyl tail; and FIG. 9, peptide-amphiphiles with three different peptide head groups. Top: Molecule 10 with "KGB". Middle: Molecule 14 lacking the phosphoserine group. Bottom: Molecule 15 with "IKVAV" SEQ ID NO 1.

SUMMARY OF THE INVENTION

In light of the foregoing, it is an object of the present invention to provide a nanostructured fiber-like system, or nanostructure providing other shapes such as spherical or oblate, and/or molecular components thereof, together with various methods for assembly and use to recreate or mimic the structural and/or functional interaction between collagen fibrils and hydroxyapatite crystals in bone or the extracellular matrix of bone prior to mineralization, thereby providing a nanostructured approach divergent from the prior art. It will be understood by those skilled in the art that one or more aspects of this invention can meet certain objectives, while one or more other aspects can meet certain other objectives. Each objective may not apply equally, in all its respects, to every aspect of this invention. As such, the following objects can be viewed in the alternative with respect to any one aspect of this invention.

It is an object of the present invention to provide a composition which can be used for assembly of a molecular structure having dimensional and functional characteristics biomimetic with collagen fibrils.

It can also be an object of the present invention to provide a nanostructured fibrous system as a template for tissue development.

It can also be an object of the present invention to provide a composite of a mineralized nanofiber structure biomimetic with collagen fibrils and hydroxyapatite crystals in natural bone tissue.

It can also be an object of the present invention to provide a system for the facile self-assembly of nanostructured fibers for use in conjunction with one or more of the preceding objectives, such fibers as can be reversibly stabilized to promote structural integrity.

It can also be an object of the present invention to provide a methodology using fibers in a stabilized three-dimensional structure to direct and/or control mineralization and crystal growth thereon.

It can also be an object of the present invention to provide a molecular system for the design and engineering of specific nanofibers and components thereof to target particular cell and/or mineral growth en route to a variety of hard or soft biomimetic materials for biological and non-biological applications, the later including, but not limited to, catalysis, photonics and electronics.

Other objects, features, benefits and advantages of the present invention will be apparent from this summary and its descriptions of various preferred embodiments, and will be readily apparent to those skilled in the art having knowledge of natural biomineralizing systems. Such objects, features, benefits and advantages will be apparent from the above as taken into conjunction with the accompanying examples, data, figures and all reasonable inferences to be drawn therefrom.

With respect to various embodiments, the present invention comprises use of self-assembly techniques, such self-assembly as may be employed in conjunction with mineralization to prepare a nanostructured composite material which recreates or mimics the structural orientation and interaction between collagen and hydroxyapatite observed in bone. A composite may be prepared by self-assembly, covalent capture, and mineralization of one or more peptide-amphiphile (PA) compositions. As evident from the preceding, the PA compositions of this invention can be synthesized using preparatory techniques well-known to those skilled in the art--preferably, by standard solid phase chemistry, with alkylation of the N-terminus of the peptide component. Mono or di-alkyl moieties attached to the N or C termini of peptides may influence their aggregation and secondary structure in water in both synthetic and natural systems. As illustrated in several embodiments, a hydrophobic, hydrocarbon and/or alkyl tail component with a sufficient number of carbon atoms coupled to an ionic peptide can be used to create an amphiphile that assembles in water into cylindrical micelles because of the amphiphile's overall conical shape. (J. N. Israelachvili Intermolecular and surface forces; 2nd ed.; Academic: London San Diego, 1992). The alkyl tails pack in the center of the micelle with the peptide segments exposed to an aqueous or hydrophilic environment. These cylindrical micelles can be viewed as fibers in which the chemistry of the peptide region is repetitively displayed on their surface. Comparably, consistent with this invention, amphiphile molecules can also be designed to provide micelles having structural shapes that may differ from a fiber like appearance.

Without limitation, three structural and/or functional features can be engineered into the peptide region of a PA composition of this invention. First, the prepared fibers are optimally robust and, for this reason, one or more consecutive cysteine amino acid residues--four in some embodiments--can be incorporated in the sequence for covalent capture of supramolecular nanofibers. (D. Y. Jackson, D. S. King, J. Chmielewski, S. Singh, P. G. Schultz, J. Am. Chem. Soc. 113, 9391-9392 (1991); T. D. Clark, K. Kobayashi, M. R. Ghadiri, Chem Eur J 5, 782-792 (1999); Y. Y. Won, H. T. Davis, F. S. Bates, Science 283, 960-963 (1999); E. R. Zubarev, M. U. Pralle, L. M. Li, S. I. Stupp, Science 283, 523-526 (1999); E. A. Archer, N. T. Goldberg, V. Lynch, J Am Chem Soc 122, 5006-5007 (2000); F. Cardullo, M. C. Calama, B. H. M. Snellink-Ruel, J. L. Weidmann, A. Bielejewska, R. Fokkens, N. M. M. Nibbering, P. Timmerman, D. N. Reinhoudt, Chem. Comm. 5, 367-368 (2000)). Such residues can be used to form disulfide bonds between adjacent PA molecules upon oxidation to lock the supramolecular structure into place. The formation of the disulfide bonds is reversible allowing self correction of improper disulfide bonds or return to the supramolecular structure by treatment with mild reducing agents.

With regard to a second feature, the fibers of various embodiments may be able to nucleate the formation of HA crystals in the proper environment. It is well known that acidic moieties play a key role in biomineralization processes and in the formation of calcium phosphate minerals phosphorylated groups are particularly important. (G. K. Hunter, H. A. Goldberg, Biochem. J. 302, 175-179 (1994); S. Weiner, L. Addadi, J. Mater. Chem. 7, 689-702 (1997)). For example in dentin, the phosphophoryn protein family contains numerous repeats of the sequences Asp-Ser(P)-Ser(P) and Ser(P)-Asp (A. George, L. Bannon, B. Sabsay, J. W. Dillon, J. Malone, A. Veis, N. A. Jenkins, D. J. Gilbert, N. G. Copeland, J. Biol. Chem. 271, 32869-32873 (1996)). These massively phosphorylated proteins are closely associated with the collagen extracellular matrix (ECM) and are known to play an important role in HA mineralization (A. Veis In Biomineralization: Chemical and Biological Perspectives; S. Mann, J. Webb, J. R. P. Williams, Eds.; VCH: Weinheim N.Y., 1989; pp 189-222). Accordingly, at least one phosphoserine residue can be incorporated into the peptide sequence which, after self assembly, allows the fiber to display a highly phosphorylated surface functionally biomimetic to a long peptide segment. This, in part, may be used to simulate a repetitive organization of phosphate groups found in phosphophoryn proteins.

Third, with respect to one or more of the preceding embodiments or others within the scope of this invention, it would be beneficial for biomedical applications to provide fibers promoting surface adhesion and growth of cells. Another collagen associated protein, fibronectin, contains the sequence Arg-Gly-Asp (RGD). As this sequence has been found to play an important role in integrin-mediated cell adhesion, an RGD sequence can also be included in preferred peptide components and/or PA compositions, depending upon end-use application. Collectively, these and other design principles led to preparation of a PA molecule of the type shown in FIG. 1.

Notwithstanding the numerous embodiments provided above, broader aspects of the present invention include a peptide amphiphile composition having 1) a hydrophobic component and 2) a peptide or peptide-like component further including a cell adhesion sequence. In various preferred embodiments, the hydrophobic component of such a composition is of sufficient length to provide amphiphilic behavior and micelle formation in water or another polar solvent system. Typically, such a component may be about a C.sub.6 or greater hydrocarbon moiety, although other hydrophobic, hydrocarbon and/or alkyl components could be used as would be well-known to those skilled in the art to provide similar structural or functional effect. Regardless, a peptide component of such a composition may include the aforementioned RGD sequence found especially useful for the nanofiber mineralization described herein.

Preferred peptide components of such compositions can also include a phosphoryl-functionalized (P) residue or sequence, as described above. Inclusion of a phosphoserine residue, S(P), has been found especially useful for HA mineralization. Other embodiments can include, for example and without limitation, a phosphotyrosine residue. The peptide component of such compositions can also include a residue or sequence capable of promoting intermolecular bonding and structural stability of the micelles/nanofibers available from such compositions. A sequence of cysteine residues can be used with good effect, providing for the facile intermolecular oxidation/reduction of the associated thiol functionalities.

Peptide components of this invention preferably comprise naturally-occurring amino acids. However, incorporation of known artificial amino acids and/or other similar monomers such as hydroxyacids are also contemplated, with the effect that the corresponding component is peptide-like in this respect. Accordingly, such artificial amino acids, hydroxyacids or related monomers can be used to meet the spacer, phosphorylation and/or intermolecular bonding objectives described above.

Various aspects of the present invention can be described with reference to the peptides amphiphile illustrated in FIG. 1, but consistent with broader aspects of this invention, other compositions can be prepared in accordance with this invention and used for the self-assembly of fibrous cylindrical micelles and corresponding nanostructures. See, Table 1, below.

TABLE-US-00001 TABLE 1 PA N-terminus Peptide (N to C) C-terminus 1 C16 SEQ ID NO 2: H CCCCGGGS(P)RGD 2 C16 SEQ ID NO 3: H CCCCGGGS(P) 3 H SEQ ID NO 4: H CCCCGGGS(P)RGD 4 C10 SEQ ID NO 5: H CCCCGGGS(P)RGD 5 C6 SEQ ID NO 6: H CCCCGGGS(P)RGD 6 C10 SEQ ID NO 7: H GGGS(P)RGD 7 C16 SEQ ID NO 8: H GGGS(P)RGD 8 C16 SEQ ID NO 9: H AAAAGGGS(P)RGD 9 C10 SEQ ID NO 10: H AAAAGGGS(P)RGD 10 C16 SEQ ID NO 11: H CCCCGGGS(P)KGE 11 C10 SEQ ID NO 12: H AAAAGGGS(P)KGE 12 C16 SEQ ID NO 13: H AAAAGGGS(P)KGE 13 C22 SEQ ID NO 14: H CCCCGGGS(P)RGD 14 C16 SEQ ID NO 15: H CCCCGGGSRGD 15 C16 SEQ ID NO 16: H CCCCGGGEIKVAV 16 C16 SEQ ID NO 17: H CCCCGGGS(P)RGDS 17 C.sub.n SEQ ID NO 18: H LLLKK-X 18 C.sub.n LSL-X H 19 C.sub.n LSLS-X H

It should be noted that within the particular system examined, Pas 3 and 5 do not exhibit micelle formation, illustrating a certain degree of hydrophobicity as may be useful in some embodiments for self-assembly of such compositions into the nanofibers or other nanostructures of this invention. Depending upon desired cell or mineral growth, a phosphorylated moiety or residue may not be included (see PAs 14 and 15). As discussed above, cellular adhesion or interaction may be promoted by a particular sequence of the peptide component. With reference to PAs 10-12 and 15, a non-RGD sequence can be utilized depending upon cellular target or end-use application. In particular, the SEQ ID NO 19 IKVAV sequence has been identified in other contexts as important for neuron growth and development. The SEQ ID NO 20 YIGSR sequence, known for a role in neuronal cell-substrate adhesion, can also be used. Accordingly, the amphiphile compositions of this invention can include a peptide component having such a sequence for corresponding use. Other residues and/or sequences capable of promoting cell adhesion, growth and/or development are known in the art and can be used in conjunction with the present invention, such residues/sequences as can be incorporated into the peptide components and/or PA compositions of this invention using available synthetic techniques or straight-forward modifications thereof. With respect to Table 1, it is noted that several PA compositions do not include cysteine residues: while such a residue or peptide sequence can be used to enhance intermolecular nanofiber stability, it is not required for micelle formation in the first instance. Reference is made to FIGS. 7-9 for TEM micrographs of several PA compositions identified in Table 1.

Further reference is made to Table 1 and, in particular, PA compositions 17-19. Various other embodiments of this invention may comprise the residues shown, or where optionally X may further comprise one or more of the aforementioned residues as may be utilized for intramolecular structural flexibility, intermolecular stability, mineralization and/or cellular interaction. Accordingly, each such composition can optionally comprise, as desired for end-use application, one or more glycine, cysteine, phosphorylated and/or cell growth, development or adhesion residues. In accordance with the preceding discussion, the amphiphilic nature of such compositions provides for the presence of a suitably hydrophobic component C.sub.n, where n is an integer corresponding to the number of carbon atoms in such a component sufficient to provide sufficient amphiphilic character and/or assembly structure. Without limitation, various embodiments of such compositions comprise a hydrophobic component of about C.sub.6-C.sub.26. More specifically and without limitation, a sequence such as that provided by PA composition 17 (or as further comprising residue(s) X) can be utilized to modify and/or enhance the rate of nanostructure self-assembly. Likewise, without limitation, the sequences of PA compositions 18 and 19 (or as further comprising residue(s) X) can be used, as needed, to improve the structural or mechanical properties of the corresponding available nanostructured gel materials. Implementation of such peptides in the PA compositions of this invention do not, of course, preclude use of other residues. For example, PA compositions 17-19 can further comprise one or more glycine, cysteine, phosphorylated and/or cellular interaction residues, in accordance with this invention.

Various peptide-amphiphile compositions are shown in Table 1, but are provided only by way of illustrating one or more aspects of this invention. It will be understood by those skilled in the art that a range of other compositions are also contemplated. For example, the peptide components can be varied, limited only by functional considerations of the sort described herein. Accordingly, peptide length and/or sequence can be modified by variation in number or identity of amino acid or substituted monomer. Further, it will be understood that the C-terminus of any such sequence can be construed in light of associated carboxylate functionality or derivative thereof. While the N-terminus of the peptides is illustrated with respect to the referenced conjugated hydrophobic and/or hydrocarbon components, it will be understood that such components can also be varied by length and/or composition, such variation limited only by the functional considerations presented herein. For example, a sixteen carbon (C16) component can comprise but is not limited to a straight-chain alkyl hydrocarbon. As would be understood by those skilled in the art, the structure and/or chemistry of such a component can be varied with regard to a particular, desired functionality of an amphiphile composition or assembly thereof. Likewise, the length of such a component is limited only by way of the degree of hydrophobicity desired for a particular amphiphile composition and/or assembled structure, in conjunction with a given solvent medium.

In part, the present invention also provides a sol-gel system comprising 1) an aqueous or polar solvent solution and/or containing one or more of the amphiphile compositions described herein, and 2) a reagent to adjust system pH. As described elsewhere herein, a system pH of less than or equal to about 4, depending on peptide identity, induces assembly, gellation or an agglomeration of the solution with such compositions--in some embodiments, biomimetic nanofibers of cylindrical micelles. Conversely, use of a suitable reagent to raise the system pH to greater than or equal to about 4 dissolves or disassociates the compositions or nanofiber micelles. In preferred embodiments, the amphiphile composition(s) of such a system includes a peptide component having residues capable of intermolecular cross-linking. The thiol moieties of cysteine residues can be used for intermolecular disulfide bond formation through introduction of a suitable oxidizing agent. Conversely, such bonds can be cleaved by a reducing agent introduced to the system.

Corresponding to one or more embodiments of such a material, composition or system, the present invention can also include a nanostructured template for mineral crystal and/or cellular growth. Such a template includes a micelle assembly of amphiphile composition(s) of the type described herein, wherein the peptide component thereof may include a residue or sequence capable of intermolecular bond formation. In preferred embodiments, as described above, cysteine residues can be used for intermolecular disulfide bond formation. Various other preferred embodiments can further include one or more phosphorylated residues to promote crystal growth and/or mineralization, depending upon a desired material or tissue target.

In the context of biomimetic hard material or tissue, the present invention can also include an organo-mineral composite having a nanostructured peptide amphiphile template with mineral crystals thereon. As described above, this aspect of the present invention can be illustrated with the present amphiphile compositions, assembled as nanostructured fibers, used to nucleate and grow hydroxyapatite crystals. In preferred embodiments, the amphiphilic compositions include peptide components having one or more residues promoting crystal nucleation and growth. Such preferred peptide components can also include one or more residues capable of intermolecular bonding to stabilize the nanofiber template. While this inventive aspect has been described in conjunction with hydroxyapatite nucleation and growth, the mineral component of this composite can include other inorganic compounds and/or oxides. Such residues, sequences or moieties are of the type described herein, or as would otherwise be understood by those skilled in the art made aware of this invention.

Regardless, the c-axes of the mineral crystals of such composites are aligned with the longitudinal fiber axes, in a manner analogous to the alignment observed between collagen fibrils and HA crystals in natural bone tissue. Accordingly, the present invention can also include a method of using a peptide amphiphile, in accordance with this invention, to promote and control HA crystal growth. The identity of the PA compositions used therewith is limited only by way of those structural considerations described elsewhere herein. Consistent therewith and with the broader aspects of this invention, such a method includes 1) providing an aqueous or other suitable polar medium of one or more peptide amphiphile compositions, 2) inducing assembly thereof into cylindrical micelle structures, 3) optimally stabilizing the structures with intermolecular bond formation, and 4) introducing to the medium reagents suitable for the preparation of HA and crystalline growth thereof on the nanofiber micelle structures. As provided elsewhere herein, one or more amphiphile compositions can be used to provide fibers with a variety of cell adhesion, mineralization and/or structural capabilities. A combination of such compositions can be used to assemble a nanofibrous matrix with synergistic properties beneficial for a particular crystal and/or cellular development, such compositions as may vary according to peptide component, residue sequence, hydrophobic or hydrocarbon component and/or resulting PA or assembly configuration.

EXAMPLES OF THE INVENTION

The following non-limiting examples and data illustrate various aspects and features relating to the compositions, micelles, composites and/or methods of the present invention, including self-assembly of a peptide-amphiphile nanofiber system, as is available through the methodologies described herein. In comparison with the prior art, the present structures, template designs and related methods provide results and data which are surprising, unexpected and contrary thereto. While the utility of this invention is illustrated through the use of several amphiphiles, biomimetic micelles and resulting organo-mineral composites, it will be understood by those skilled in the art that comparable results are obtainable with various other amphiphiles, nanofibers/micelles and/or composites, as are commensurate with the scope of this invention.

Example 1

After synthesis (see, example 10), the PA of FIG. 1 (characterized by .sup.1H NMR and MALDI-TOF MS: [M-H].sup.-1=1333) was treated with dithiothreitol (DTT) at a pH of 8 to reduce all cysteine residues to free thiols. At this pH the PA was found to be soluble in excess of 50 mg/ml in water. However, upon acidification of the solution below pH 4 the material rapidly becomes insoluble. Solutions more concentrated than 2.5 mg/ml form birefringent gels in water that are self-supporting upon inversion of the container. Examination of the gels by cryo-TEM, which preserves the native, hydrated state of the material, revealed a network of fibers with a diameter of 7.6.+-.1 nm and lengths up to several microns (FIG. 2B). Negatives were digitized in the Umax PowerLook scanner with resolution 1200 dpi. The average thickness of the PA fibers was determined by two different procedures. First the Fourier transform of the images and following measurements of the distances between the peaks were performed in the NIH Image 1201.1262 program. The second approach applied was averaging technique based on the cross-correlation method, widely used in single particle reconstruction using the EMAN 1201.1202 program. For each data set 1200-1300 individual boxes were selected. (E. Beniash, W. Traub, A. Veis, S. Weiner, J. Struct. Biol. 132, 212-225 (2000). Positively and negatively stained dried fibers were found to have diameters of 6.0.+-.1 nm (FIGS. 2A,C). TEM using the positive stain uranyl acetate, which preferentially stains acidic groups, revealed increased electron density at the periphery of the fiber. (J. R. Harris, Ed. Electron Microscopy in Biology, A Practical Approach; Oxford University Press: New York, 1991). Additionally, gels that were stained, embedded in epoxy resin and sectioned for TEM, showed fibers in cross-section in which donut shaped patterns were observed indicating staining only on the outer portion of the fiber (FIG. 2D). The two positive staining experiments of this example indicate that the hydrophobic alkyl tails pack on the inside of the fiber and the acidic moieties of the peptide are displayed on the surface of the fiber.

Example 2

The formation of the fibers was found to be concentration independent over more than three orders of magnitude (0.01 mg/ml to 50 mg/ml), however a second level of hierarchy was observed that was concentration dependent. As the concentration of the PA was increased, a larger number of the fibers were observed to pack into flat ribbons of fibers (FIGS. 2A,C).

Example 3

Examination of the self-assembled material by FT-IR revealed a bimodal amide I peak with maxima at 1658 cm.sup.1-(.alpha.-helix) and 1632cm.sup.-1 (.beta.-sheet) along with a N--H stretching peak at 3287 cm.sup.-1 indicating the formation of a hydrogen bonded structure, possibly utilizing a combination of .beta.-sheet and .alpha.-helical secondary structure in the fibers (S. Krimm, J. Bandekar, Adv. Protein Chem. 38, 181-364 (1986); W. K. Surewicz, H. H. Mantsch, D. Chapman, Biochemistry 32, 389-394 (1993)). Based on the above data the nanofibers were modeled as cylindrical micelles in which the alkyl tails pack on the inside of the fiber and peptide segments are displayed on the outside containing both .beta.-sheet and .alpha.-helical secondary structure. This model results in a fiber with a diameter of 8.5 nm, which is within the margin of error of our TEM measurements (FIG. 1C).

Example 4

Upon lowering the pH below 4 with HCl the material self-assembles; when the pH is brought back to neutrality with KOH it disassembles. This pH triggered self-assembly and gelation allows the material to respond to its environment and thus may have applications in controlled release of molecules (W. A. Petka, J. L. Hardin, K. P. McGrath, D. Wirtz, D. A. Tirrell, Science 281, 389-392 (1998)).

Example 5

Following self-assembly of PA molecules into fibers, the cysteine thiol groups were oxidized by treatment with 0.01M iodine. Examination of the material by TEM reveals that the fibers remain intact (FIGS. 2C,D). These oxidized PA fibers were found to be stable to alkaline solutions (pH 8) for months while fibers which had not been oxidized would disassemble within minutes. Based on the length of the fibers revealed by TEM and the present model for molecular organization of fibers, the resulting polymer has a molecular weight of roughly 2.times.10.sup.8 daltons. Importantly, the covalent capture process of this invention can be easily reversed by treating the fibers with a mild reducing agent such as DTT. After reduction the fibers regain their pH sensitivity and rapidly disassemble at pH 8. Together with the controlled self-assembly, reversible cross-linking provides a highly dynamic system which can interconvert between discrete molecules, self-assembled supramolecular fibers, covalently captured polymeric fibers and back again to single small molecules depending solely on the environment in which the material is placed.

Example 6

To investigate the mineralization properties of PA nanofibers of this invention, the material was assembled and mineralized directly on a holey carbon coated TEM grid--to allow study of the dynamics of the mineralization process while minimizing artifacts of TEM sample preparation. To prepare samples, a drop of aqueous PA (1 mg/ml) was mounted on the holey grid and the self-assembly of the PA was induced in an atmosphere of HCl vapor. The grids were immersed in aqueous iodine to oxidize the cysteine thiol groups to disulfides. After rinsing with distilled water, the PA coated grids were treated with 5 .mu.l of 10 mM CaCl.sub.2 on one side and 5 .mu.l of 5 mM Na.sub.2HPO.sub.4 on the other side. The two solutions are able to mix only by passing through the holes in the carbon support. Grids were examined by TEM at different time intervals (FIG. 3) and after 10 minutes inorganic material was observed to be concentrated around the fibers thus increasing their contrast (a crystalline phase was not detected at this time by electron diffraction). At 20 minutes the fibers begin to be covered with crystalline mineral, although significant amourphous material remains. After 30 minutes plate shaped polycrystalline mineral is visible throughout the surface of the fibers (FIG. 3C and FIG. 5). The mineral was analyzed by EDS (Energy Dispersion X-Ray Fluorescence Spectroscopy) which revealed a Ca/P ratio of 1.67.+-.0.08 which is consistent with the formation of HA with a formula of Ca.sub.10(PO.sub.4).sub.6(OH).sub.2 (FIG. 3F).

Example 7

As controls for the experiment of example 6, carbon coated TEM grids were treated as above but without PA fibers. In this case no mineral deposit was found on the grids. In a second control, a PA was prepared in which phosphoserine was replaced by serine and treated as above with calcium and phosphate. The nonphosphorylated fibers were observed by TEM after 20 and 30 minutes of incubation and in both cases an amorphous mineral deposit around the fibers was observed, but crystals did not form within this time frame (FIG. 6).

Example 8

In order to discern the relative orientation of the HA crystals with respect to PA fibers, several samples of example 6, in which isolated mineralized bundles of PA fibers could be observed, were analyzed by electron diffraction. In all cases preferential alignment of the HA crystallographic c-axis with the PA fiber long axis was observed. (FIGS. 3D,E).

Example 9

Mineralization experiments show that PA fibers of this invention are able to nucleate hydroxyapatite on their surfaces. Negatively charged surfaces can promote mineralization by establishing local ion supersaturation (S. Weiner, L. Addadi, J. Mater. Chem. 7, 689-702 (1997)). Particularly, the two acidic aminoacids phosphoserine and aspartic acid used here are abundant in the proteins of mineralized tissues proven to initiate crystal growth (L. Addadi, S. Weiner, Proc. Natl. Acad. Sci. 82, 4110-4114 (1985); G. Falini, S. Albeck, S. Weiner, L. Addadi, Science 271, 67-69 (1996); A. George, L. Bannon, B. Sabsay, J. W. Dillon, J. Malone, A. Veis, N. A. Jenkins, D. J. Gilbert, N. G. Copeland, J. Biol. Chem. 271, 32869-32873 (1996); S. Weiner, L. Addadi, J. Mater. Chem. 7, 689-702 (1997)). The fact that the fibers gain extra electron density prior to formation of the crystalline phase suggests the above mechanism may be utilized in our system. More surprising is the observation that the c-axes of the HA crystals are co-aligned with long axes of the fibers (FIG. 4). This fact implies that the orientation of crystalline nuclei and the subsequent crystal growth are not random but are controlled by the PA micelles. The exact mechanism of this control is not clear, however in similar systems such control is gained by specific arrangement of acidic groups that promote growth of the crystals in a particular orientation by an epitaxial mechanism (S. Mann, J. P. Hannington, R. J. P. Williams, Nature 324, 565-567 (1986); S. Weiner, L. Addadi, J. Mater. Chem. 7, 689-702 (1997); J. Aizenberg, A. J. Black, G. M. Whitesides, Nature 398, 495-498 (1999)). An analogous mechanism may be employed with PA fibers. Previous in vitro studies showing oriented crystal growth from organic templates were done mainly in two dimensional systems. The results of this example show for the first time oriented crystal growth in a synthetic fiberous organic substrate.

Example 10

Several representative peptide-amphiphiles of this invention were prepared by manual solid phase peptide synthesis starting from 0.5 mmoles of an FMOC-Asp(tBu)-WANG resin. Deprotection of the initial and subsequent FMOC groups and was accomplished by double treatment with 15 ml of 30% piperidine in DMF for 2 minutes and 7 minutes. The resin was then washed with 15 ml of DMF 5 times. With the exception of cysteine derivatives, amino acids (4 equivalents) were preactivated with HBTU (3.95 equivalents) and DiEA (6 equivalents) in 10 ml of DMF for two minutes. The activated solution was then added to the deprotected resin and allowed to shake for 30 minutes after which a small sample was removed and analyzed with ninhydrin to test for completeness of the reaction. Failed reactions were recoupled in an identical fashion. Cysteine was coupled via the FMOC-Cys(Trt)-OPfp activated ester with the addition of 1 equivalent of DiEA in order to avoid suspected epimerization found using the standard FMOC-Cys(ACM)-OH derivative with the conditions described above. Other amino acid derivatives used included FMOC-Gly-OH, FMOC-Arg(Pbf)-OH and FMOC-Ser(PO(OBzl)OH)-OH. Analogous derivatives available in the art may be used to couple A, S, L, K and other such residues, including those shown in Table 1. Protecting groups may vary depending on the subject amino acid residue. The final step was coupling of a suitable fatty acid or derivative thereof (e.g., palmitic acid) and was accomplished using 2 equivalents of the acid activated with 2 equivalent of HBTU and 3 equivalents of DiEA in 15 ml DMF for 2 minutes. The solution was allowed to react with the resin overnight. Likewise, other reagents and starting materials of the sort useful in the preparation of the inventive compositions, including but not limited to those shown in Table 1, are readily-available and known to those skilled in the art, such reagents/starting materials as may be used for the hydrophobic/hydrocarbon and peptide (residues/monomers) components.

Cleavage and deprotection of the peptide-amphiphiles was done with a mixture of TFA, water, triisopropyl silane (TIS) and ethane dithiol (EDT) in a ratio of 91:3:3:3 for three hours at room temperature. The cleavage mixture and TFA washings were filtered into a round bottom flask. The solution was roto-evaporated and then redissolved in a minimum of neat TFA. This solution was triturated with cold diethylether. The white precipitation was collected by filtration and dried under vacuum.

Various other amphiphile compositions of this invention can be prepared in analogous fashion, as would be known to those skilled in the art and aware thereof, using the above-described known procedures and synthetic techniques or straight-forward modifications thereof depending upon a desired amphiphile composition or peptide sequence.

As demonstrated by the preceding examples, figures and data, the fact that the hydroxyapatite crystals grow on the bundles of fibers with their c-axes oriented along the long axes of the micelles could be of interest for design of new materials for mineralized tissue repair. This nanoscale organization resembles that of hydroxyapatite crystals in mineralized ECM in which the HA crystals also grow in parallel arrays with their c-axes co-aligned with long axes of the organic fibers (W. Traub, S. Weiner, Proc. Nat. Acad. Sci. 86, 9822-9826 (1989)). This arrangement is the most important characteristic of the biominerals belonging to the bone family (S. Weiner, H. D. Wagner, Annu. Rev. Mater. Sci. 28, 271-298 (1998)). The organization of the collagen fibers, porosity, mineral-organic ratio vary in different members of this family, yet all of them are built from the collagen fibrils containing parallel arrays of hydroxyapatite crystals (W. Traub, S. Weiner, Proc. Nat. Acad. Sci. 86, 9822-9826 (1989); S. Weiner, W. Traub, FASEB J. 6, 879-885 (1992); W. J. Landis, K. J. Hodgens, J. Arena, M. J. Song, B. F. MeEwen, Microsc. Res. Techniq. 33, 192-202 (1996)).

While the principles of this invention have been described in connection with specific embodiments, it should be understood clearly that these descriptions are added only by way of example and are not intended to limit, in any way, the scope of this invention. For instance, various peptide amphiphiles have been described in conjunction with specific residues and corresponding cell adhesion, but other residues can be used herewith to promote a particular cell adhesion and tissue growth on the nanostructures prepared therefrom. Likewise, while the present invention has been described as applicable to biomimetic material or tissue engineering, it is also contemplated that gels or related systems of such peptide amphiphiles can be used as a delivery platform or carrier for cells or cellular material incorporated therein. Other advantages and features will become apparent from the claims filed hereafter, with the scope of such claims to be determined by their reasonable equivalents, as would be understood by those skilled in the art.

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25mo sapiens s Val Ala ValRTHomo sapiensS(8)..(8)phosphorylated serine 2Cys Cys Cys Cys Gly Gly Gly Ser Arg Gly Asp8PRThomo sapiensS(8)..(8)phosphorylated serine 3Cys Cys Cys Cys Gly Gly Gly SerRThomo sapiensS(8)..(8)phosphorylated serine 4Cys Cys Cys Cys Gly Gly Gly Ser Arg Gly Aspmo sapiensS(8)..(8)phosphorylated serine 5Cys Cys Cys Cys Gly Gly Gly Ser Arg Gly Aspmo sapiensS(8)..(8)phosphorylated serine 6Cys Cys Cys Cys Gly Gly Gly Ser Arg Gly Asp7PRThomo sapiensS(4)..(4)phosphorylated serine 7Gly Gly Gly Ser Arg Gly AspThomo sapiensS(4)..(4)phosphorylated serine 8Gly Gly Gly Ser Arg Gly AspRThomo sapiensS(8)..(8)phosphorylated serine 9Ala Ala Ala Ala Gly Gly Gly Ser Arg Gly Aspomo sapiensS(8)..(8)phosphorylated serine la Ala Ala Gly Gly Gly Ser Arg Gly Aspomo sapiensS(8)..(8)phosphorylated serine ys Cys Cys Gly Gly Gly Ser Lys Gly Glu2mo sapiensS(8)..(8)phosphorylated serine la Ala Ala Gly Gly Gly Ser Lys Gly Glu3mo sapiensS(8)..(8)phosphorylated serine la Ala Ala Gly Gly Gly Ser Lys Gly Glu4mo sapiensS(8)..(8)phosphorylated serine ys Cys Cys Gly Gly Gly Ser Arg Gly Asp5mo sapiens ys Cys Cys Gly Gly Gly Ser Arg Gly Asp6mo sapiens ys Cys Cys Gly Gly Gly Glu Ile Lys Val Ala Val7mo sapiensS(8)..(8)phosphorylated serine ys Cys Cys Gly Gly Gly Ser Arg Gly Asp Ser88PRThomo sapiensX(6)..(8)Xaa can be Gly or any one other naturally occurring amino acid; some residue information may be missing from Xaa. eu Leu Lys Lys Xaa Xaa XaaRThomo sapiens ys Val Ala ValRThomo sapiens 2e Gly Ser ArgRThomo sapiens 2s Val Ala ValRThomo sapiens 22Leu Leu Leu Lys LysRThomo sapiens 23Ala Ala Ala Ala Gly Gly GlyRThomo sapiens 24Ile Lys Val Ala ValRThomo sapiens 25Tyr Ile Gly Ser Arg>
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