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United States Patent | 5,639,949 |
Ligon , et al. | June 17, 1997 |
The present invention is directed to the production of an antipathogenic substance (APS) in a host via recombinant expression of the polypeptides needed to biologically synthesize the APS. Genes encoding polypeptides necessary to produce particular antipathogenic substances are provided, along with methods for identifying and isolating genes needed to recombinantly biosynthesize any desired APS. The cloned genes may be transformed and expressed in a desired host organisms to produce the APS according to the invention for a variety of purposes, including protecting the host from a pathogen, developing the host as a biocontrol agent, and producing large, uniform amounts of the APS.
Inventors: | Ligon; James M. (Basel, CH), Hill; Dwight Steven (Cary, NC), Ryals; John Andrew (Durham, NC), Lam; Stephen Ting (Raleigh, NC), Hammer; Philip E. (Cary, NC) |
Assignee: |
Ciba-Geigy Corporation
(Tarrytown,
NY)
|
Appl. No.: | 08/258,261 |
Filed: | June 8, 1994 |
Application Number | Filing Date | Patent Number | Issue Date | ||
937648 | Aug., 1992 | ||||
87636 | Jul., 1993 | ||||
908284 | Jul., 1992 | ||||
570184 | Aug., 1990 | ||||
Current U.S. Class: | 800/301 ; 435/117; 435/252.33; 435/252.34; 435/320.1; 435/69.1; 435/71.1; 536/23.2; 536/23.7 |
Current International Class: | C12N 15/52 (20060101); C12N 15/78 (20060101); C07K 14/21 (20060101); C07K 14/195 (20060101); C12N 15/74 (20060101); C12N 15/82 (20060101); A01H 005/00 (); C12N 015/31 (); C12N 015/82 (); C12P 017/00 (); C12R 001/38 () |
Field of Search: | 536/23.2,23.7 435/69.1,70.1,117,172.3,320.1,252.33,252.34,71.1 800/205 |
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9208355 | May., 1992 | WO | |||
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