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United States Patent | 4,440,859 |
Rutter , et al. | April 3, 1984 |
Microorganism having a gene derived from a higher organism is produced by isolating cells from a higher organism containing messenger RNA, extracting the messenger RNA, synthesizing a double stranded cDNA using the messenger RNA as a template, inserting the cDNA into a plasmid and transforming a microorganism with the resultant recombinant plasmid.
Inventors: | Rutter; William J. (San Francisco, CA), Goodman; Howard M. (San Francisco, CA), Ullrich; Axel (San Francisco, CA), Shine; John (San Francisco, CA), Chirgwin; John (San Francisco, CA), Pictet; Raymond (San Francisco, CA) |
Assignee: |
The Regents of the University of California
(Berkeley,
CA)
|
Appl. No.: | 06/301,141 |
Filed: | September 11, 1981 |
Application Number | Filing Date | Patent Number | Issue Date | ||
801343 | May., 1977 | ||||
Current U.S. Class: | 435/91.41 ; 435/320.1; 435/488; 435/69.1; 435/69.4 |
Current International Class: | C12N 15/00 (20060101); C12N 015/00 (); C12P 021/00 (); C12P 021/04 (); C12P 019/34 (); C12N 001/00 () |
Field of Search: | 435/172,68,70,71,91 |
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